摘要
以木犀草素为模板分子,α-甲基丙烯酸(α-methyl acrylic acid,MAA)为功能单体,乙二醇二甲基丙烯酸酯(ethylene glycol dimethacrylate,EGDMA)为交联剂,通过沉淀聚合法制备木犀草素分子印迹聚合物;并以其为固相萃取柱填料,制备木犀草素分子印迹固相萃取柱,确定该固相萃取柱的最佳吸附条件;用此固相萃取柱对花生壳粗提物中的木犀草素进行分离纯化。结果表明,该固相萃取柱对木犀草素表现出较好的特异性吸附效果,可分离纯化出相对纯度为94.18%的木犀草素。
Molecularly imprinted polymers(MIPs) specific for luteolin were prepared via a precipitation polymerization process that utilized luteolin,α-methyl acrylic acid(MAA),and ethylene glycol dimethacrylate(EGDMA)as template molecules,functional monomers,and crosslinking agents,respectively. The molecularly imprinted polymers were chosen as packing material for a solid-phase extraction column. The optimal adsorption conditions for this solid-phase extraction column were determined by using the prepared column to separate and purify luteolin from crude peanut shell extracts. Results showed that the solid-phase column specifically adsorbed luteolin and separated it to a relative purity of 94.18%.
作者
刘展眉
张汉辉
东方云
肖裕泽
程杏安
蒋旭红
LIU Zhan-mei;ZHANG Han-hui;DONG Fang-yun;XIAO Yu-ze;CHENG Xing-an;JIANG Xu-hong(Department of Teaching and Research,Guangzhou Nanyang Polytechnic College,Guangzhou 510900,Guangdong,China;College of Chemistry and Chemical Engineering,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,Guangdong,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第16期92-98,共7页
Food Research and Development
基金
国家自然科学基金项目(21376281)
广东省普通高校省级重点科研项目(2018GKZDXM010)。
关键词
木犀草素
沉淀聚合法
分子印迹聚合物
固相萃取
luteolin
precipitation polymerization
molecularly imprinted polymers(MIPs)
solid-phase extraction
