IFITM3基因单核苷酸多态性与急性淋巴细胞白血病关系

Relationship between single nucleotide polymorphism of gene IFITM3 and acute lymphoblastic leukemia

目的探讨急性淋巴细胞白血病(ALL)患者干扰素(IFN)诱导跨膜蛋白3(IFITM3)的单核苷酸多态性(SNP)与其发病的相关性,为ALL临床预防和诊断提供依据。方法选取2018-01-06-2019-02-06本院收治的60例ALL患者为病例组,另选取同期本院健康体检者50名作为对照组,比较2组IFITM3基因的SNP,分析其与抗ALL的DNA抗体、C3和C4补体的相关性。结果病例组IFITM3表达量为6.77±2.03,高于对照组的3.02±0.59,t=12.617,P<0.001。病例组抗ALL的DNA抗体水平为(1.01±0.24)mg/L,低于对照组的(3.24±1.29)mg/L,t=13.132,P<0.001;C3补体水平为(2.68±1.27)g/L,高于对照组的(1.03±0.54)g/L,t=8.560,P<0.001;C4补体水平为(1.58±0.35)g/L,高于对照组的(0.77±0.04)g/L,t=16.264,P<0.001。病例组GA和GG例数多于对照组,AA例数少于对照组,差异有统计学意义,χ^(2)=54.797,P<0.001。病例组G等位基因频率为70.00%,大于对照组的36.00%,χ^(2)=10.897,P<0.001。结论IFITM3基因多态性与ALL有关,携带GA和GG基因型者发病风险增大。

Objective To investigate relationship between single nucleotide polymorphism(SNP)of gene interferon-induced transmembrane protein 3(IFITM3)and pathogenesis of acute lymphoblastic leukemia(ALL),and to provide valuable information for further improving the clinical prevention and diagnosis of ALL.Methods Sixty ALL patients and fifty healthy individuals in our hospital from January 6,2018 to February 6,2019 were enrolled,and set as case group and control group,respectively.SNP of IFITM3 gene was compared between groups,and its correlation with anti-ALL DNA antibodies,C3 complement,C4 complement and genetic susceptibility was analyzed.Results The expression of IFITM3 in case group was higher than that in control group(6.77±2.03 vs 3.02±0.59),t=12.617,P<0.001.The DNA antibody level against ALL in the case group was(1.01±0.24)mg/L,which was lower than the control group’s(3.24±1.29)mg/L,t=13.132,P<0.001.The C3 complement level was(2.68±1.27)g/L,higher than the control group’s(1.03±0.54)g/L,t=8.560,P<0.001.The C4 complement level was(1.58±0.35)g/L,higher than the control group’s(0.77±0.04)g/L,t=16.264,P<0.001.The number of GA and GG cases in the case group were more than that in the control group,and the number of AA cases was less than that in the control group.The difference was statistically significant,χ^(2)=54.797,P<0.001.The frequency of G allele in case group was higher than that in control group(70.00%vs36.00%),χ^(2)=10.897,P<0.001.Conclusion IFITM3 expression is related to ALL at the gene level,and the occurrence and development of ALL can be predicted and diagnosed by detecting SNP of IFITM3 gene in peripheral blood.