小分子药物传输载体综合实验设计

Integrated experiment design of small molecule drug delivery carrier

将较为成熟的科研实例小分子药物传输载体聚乙二醇-聚己内酯(PEG-PCL)设计为高年级本科生的综合实验,探索促进科教融合的新途径和新方法。采用辛酸亚锡催化端羟基聚乙二醇(PEG)引发己内酯开环聚合制备PEG-PCL,合成方法具有简便易行和成本较低的特点。通过自组装方法负载小分子药物,并结合聚合物和纳米材料表征手段以及生物医学评价技术,涵盖开环聚合等高分子化学、自组装等高分子物理、核磁和电镜分析技术以及纳米药物-细胞相互作用评价方法等多学科知识与技能,使学生全面了解生物材料研发的完整流程,启发和训练学生系统掌握小分子药物传输载体的研究能力。

Amphiphilic copolymer pegylated-polycaprolactone(PEG-PCL)is synthesized via ring-open polymerization using stannous octoate(Sn(Oct)2)as catalyst,which is a feasible comprehensive experiment for senior undergraduates.DOX encapsulated nanomedicine is prepared by hydrophobic interaction-based assembly with copolymer PEG-PCL and small molecule drug doxorubicin(DOX).Some characterization approaches and biomedical analysis methods are used to evaluation the performances of polymer and nanomedicine.This experiment uses multidisciplinary research methods including polymeric chemistry(e.g.,ring-open polymerization),polymeric physic(e.g.,self-assembly),polymer characterization,and biomedical analyses,which is favor of training students’research ability and learning the knowledge and skills about design,fabrication,and functional evaluation of biomaterials.