摘要
By re-analzying public metagenomic data from 101 patients infected with influenza A virus during the 2007–2012 H1N1 flu seasons in France,we identified 22 samples with SARS-CoV sequences.In three of them,the SARS genome sequences could be fully assembled out of each.These sequences are highly similar(99.99%and 99.70%)to the artificially constructed recombinant SARS-CoV(SARSr-CoV)strains generated by the J.Craig Venter Institute in the USA.Moreover,samples from different flu seasons have different SARS-CoV strains,and the divergence between these strains cannot be explained by natural evolution.Our study also shows that retrospective studies using public metagenomic data from past major epidemic outbreaks serve as a genomic strategy for the research of the origins or spread of infectious diseases.
基金
supported by the National Key R&D Program of China(2021YFC0863400)
the Key Program of Chinese Academy of Sciences(KJZD-SW-L14)
the National Natural Science Foundation of China(Grant No.31571370 and 91731302).
