地肤子总黄酮对对乙酰氨基酚所致小鼠急性肝损伤的保护作用

Protective effects of flavonoid of Fructus Kochiae on Paracetamol-induced acute liver injury in mice

目的探讨地肤子总黄酮(FFK)对对乙酰氨基酚致小鼠急性肝损伤的保护作用。方法选取一月龄SPF级昆明种小鼠36只,体重(20±2)g,雌雄各半,按随机数字表法将其分为FFK低、中、高剂量组(75、150、300 mg/kg)和阳性药对照组(联苯双酯,150 mg/kg)、空白对照组(等量生理盐水)、对乙酰氨基酚模型组(等量生理盐水),每组6只。连续灌胃给药7 d后,空白对照组腹腔注射生理盐水(0.1 ml/10 g),其余各组腹腔注射对乙酰氨基酚(350 mg/kg)建立小鼠急性肝损伤模型。24 h后,检测各组血清中的谷丙转氨酶(GPT)、谷草转氨酶(GOT)及肝脏组织中的脂质过氧化物丙二醛(MDA)、超氧化物歧化酶(SOD)含量,采用苏木精-伊红(HE)染色观察各组肝脏组织病理学变化。结果对乙酰氨基酚模型组GPT、GOT、MDA高于空白对照组,SOD低于空白对照组,差异有高度统计学意义(P<0.01)。FFK低剂量组各指标与对乙酰氨基酚模型组比较,差异无统计学意义(P>0.05)。FFK中、高剂量组GPT、GOT、MDA低于对乙酰氨基酚模型组和FFK低剂量组,SOD高于对乙酰氨基酚模型组和FFK低剂量组,差异有统计学意义(P<0.05)。病理切片的HE染色结果显示除空白对照组外,其他各组可见不同程度的肝损伤。结论一定剂量的FFK对于对乙酰氨基酚致小鼠急性肝损伤具有较好的保护作用,其增强肝脏组织SOD活性,减少MDA堆积的能力可能是保肝降酶的作用机制之一。

Objective To observe the protective effects of flavonoid of Fructus Kochiae(FFK)on Paracetamol-induced acute liver injury in mice.Methods Thirty-six one-month old SPF Kunming mice with body weight of(20±2)g were selected,male and female half,they were divided into FFK low-dose,medium-dose,and high-dose groups(75,150,300 mg/kg)and positive control group(Bifendate,150 mg/kg),blank control group(equal amount of normal saline),and Paracetamol model group(equal amount of normal saline),with six mice in each group.After seven days of continuous intragastric administration,the blank control group was intraperitoneally injected with normal saline(0.1 ml/10 g),and the other groups were intraperitoneally injected with Paracetamol(350 mg/kg)to establish acute liver injury model.After 24 hours,glutamic-pyruvic transaminase(GPT)and glutamic-oxaloacetic transaminase(GOT)levels in serum and the contents of lipid peroxides malondialdehyde(MDA)and superoxide dismutase(SOD)in liver tissues were detected,and the histopathological changes of liver tissues were observed by hematoxylin-eosin(HE)staining.Results The GPT,GOT,and MDA in Paracetamol model group were higher than those in blank control group,while SOD was lower than blank control group,the differences were highly statistically significant(P<0.01).There was no significant difference between FFK low-dose group and Paracetamol model group(P>0.05).The GPT,GOT and MDA in medium-dose and high-dose FFK groups were lower than those in Paracetamol model group and low-dose FFK group,while SOD was higher than those in Paracetamol model group and low-dose FFK group,with statistical significance(P<0.05).HE staining results of pathological sections showed different degrees of liver injury in all groups except blank control group.Conclusion A certain does of FFK has a good protective effect on Paracetamol-induced acute liver injury in mice,and its ability to enhance SOD activity in liver tissue and reduce MDA accumulation may be one of the mechanisms of its liver-protecting and lowering enzymes.