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柴胡疏肝散对早期肝纤维化大鼠JAK2/STAT3信号通路的影响 被引量:3

Effects of Chaihushugan powder on the JAK2/STAT3 signaling pathway in rats with hepatic fibrosis
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摘要 目的:研究柴胡疏肝散对早期肝纤维化大鼠JAK2/STAT3信号通路的影响。方法:以CCl4诱导造模,将SD大鼠50只按随机数字表法分为正常组(腹腔注射橄榄油+蒸馏水灌胃),模型组(腹腔注射40%CCl4橄榄油溶液,蒸馏水灌胃),柴胡疏肝散低、中、高剂量组(腹腔注射40%CCl4橄榄油溶液,同时分别按生药3.5,6.3,12.6 g/kg灌胃)。柴胡疏肝散给药至第10周,观察大鼠大体形态,计算肝系数;全自动生化分析仪测定血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST),酶联免疫吸附法(ELISA)检测血清透明质酸(HA)、Ⅲ型前胶原(PCⅢ)、Ⅳ型胶原(Ⅳ-C)变化;HE染色观察各组大鼠肝脏纤维化程度;荧光定量PCR(qPCR)法检测肝组织中的JAK2、STAT3 mRNA表达;Western blot法检测肝组织中JAK2和STAT3蛋白表达。结果:与正常组比较,模型组大鼠血清ALT、AST、HA、PCⅢ、Ⅳ-C的含量均明显升高(P<0.01),HE染色显示模型组大鼠肝细胞出现明显水肿、坏死、炎性细胞浸润。与模型组比较,柴胡疏肝散低、中、高剂量组能抑制肝细胞坏死、炎性细胞浸润,且血清中ALT、AST、HA、PCⅢ、Ⅳ-C水平均显著降低(P<0.05或P<0.01);柴胡疏肝散中、高剂量组大鼠的肝系数显著降低(P<0.05);柴胡疏肝散各剂量组不同程度抑制肝组织中JAK2、STAT3 mRNA及蛋白的表达(P<0.05或P<0.01)。结论:柴胡疏肝散对早期肝纤维化大鼠的保护作用可能与抑制JAK2/STAT3信号通路相关。 OBJECTIVE:To study effects of Chaihushugan powder on the JAK2/STAT3 signaling pathway in rats with early liver fibrosis.METHODS:Fifty SD rats were treated with CCl;and randomly divided into normal group(intraperitoneal injection of olive oil+intragastric administration of distilled water),model group(intraperitoneal injection of 40%CCl;olive oil solution+intragastric administration of distilled water),and low,medium and high dose groups of Chaihushugan powder(intraperitoneal injection of 40%CCl;olive oil solution+intragastric administration of 3.5,6.3 and 12.6 g/kg crude drugs,respectively).After administration of Chaihushugan powder until the tenth week,general morphology was observed,and liver indices were measured.ALT and AST were determined by automatic biochemical analyzer.Changes of serum(HA,PCⅢ,Ⅳ-C)were detected by ELISA.Pathological changes of the hepatic fibrosis tissues in each group were observed by HE staining.mRNA expressions of JAK2 and STAT3 in liver tissues were detected by qPCR,and protein expressions of JAK2 and STAT3 in liver tissue were detected by Western blot.RESULTS:Compared with the normal group,levels of serum ALT,AST,HA,PCⅢ,Ⅳ-C in the model group were significantly increased(P<0.01).HE staining showed obvious edema of liver cells,with increased volume and inflammatory cell infiltration.Compared with the model group,different doses of Chaihushugan powder inhibited hepatocyte necrosis and inflammatory cell infiltration,and serum levels of ALT,AST,HA,PCⅢ,andⅣ-C were significantly reduced(P<0.05 or P<0.01).The liver indices of rats in the middle and high dose groups of Chaihushugan powder decreased significantly(P<0.05).Each dose group of Chaihushugan powder inhibited the expression of JAK2,STAT3 mRNA and protein in liver tissues(P<0.05 or P<0.01).CONCLUSION:The protective effect of Chaihushugan powder on early hepatic fibrosis rats might be related to inhibition of the JAK2/STAT3 signaling pathway.
作者 杨武斌 唐金凤 米本中 YANG Wubin;TANG Jinfeng;MI Benzhong(Clinical Pharmacy,Chongqing Gaoxin District People's Hospital,Chongqing 400039;Key Laboratory of Birth Defects and Reproductive Health of National Health Commission,Chongqing Population and Family Planning Science and Technology Research Institute,Chongqing 400020;Institute of Medical Laboratory Animals,Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)
出处 《癌变.畸变.突变》 CAS 2022年第3期200-205,共6页 Carcinogenesis,Teratogenesis & Mutagenesis
基金 重庆市科卫联合中医药科技项目(ZY201802125)。
关键词 柴胡疏肝散 肝纤维化 JAK2/STAT3信号通路 保护作用 Chaihushugan powder hepatic fibrosis JAK2/STAT3 signal pathway protective effect
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