Mechanism of Olibanum and Myrrha for the Acute Soft Tissue Injury Based on Network Pharmacology

基于网络药理学探讨乳香、没药治疗急性软组织损伤机制

Objective The objective of this study was to screen the therapeutic target of olibanum and myrrha on acute soft tissue injury(ASTI)by network pharmacology and to clarify their mechanisms.Methods The main chemical constituents and the targets of olibanum and myrrha were obtained by using traditional Chinese medicine systems pharmacology database and analysis platform database.The disease targets of ASTI were searched by GeneCards.The intersection targets of herbs and diseases were selected for protein interaction analysis,protein–protein interaction network was constructed,and potential protein functional modules in the network were explored.A compound–target–disease network was constructed using Cytoscape3.8.2 software.The targets were analyzed by gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes enrichment analysis based on the Metascape database.Results The core active components of olibanum and myrrha were quercetin,β-sitosterol,and stigmasterol.The core targets were PGR,NCOA2,PTGS2,PRKCA,and NR3C2.Pathways in cancer,AGE-RAGE signaling pathway in diabetic complications might play a potential role in olibanum and myrrha in the treatment of ASTI.Conclusion Olibanum and myrrha have the characteristics of multiple components,multiple targets,and overall regulation in the treatment of ASTI.

目的:应用网络药理学方法,筛选乳香、没药治疗急性软组织损伤的靶点,并阐明其作用机制。方法:利用TCMSP数据库获取乳香、没药的主要化学成分及其靶点;在GeneCards数据库中检索急性软组织损伤的疾病靶点;选取乳香、没药与急性软组织损伤的交集靶点进行蛋白质相互作用分析,构建蛋白质相互作用网络,并挖掘网络中潜在的蛋白质功能模块;应用Cytoscape3.8.2软件构建成分—靶点—疾病网络;基于Metascape数据库对所筛选靶点进行GO分析和KEGG富集分析。结果:乳香、没药的核心活性成分为槲皮素、β-谷甾醇、豆甾醇。核心靶点为PGR、NCOA2、PTGS2、PRKCA和NR3C2。肿瘤信号通路及糖尿病并发症的AGE-RAGE信号通路可能在乳香、没药治疗急性软组织损伤的信号通路中发挥潜在作用。结论:乳香、没药治疗急性软组织损伤具有多组分、多靶点及整体调节的特点。