血清非编码RNA Linc00673在胃癌患者诊断中的临床应用

Clinical application of serum long non-coding RNA Linc00673 in the diagnosis of gastric cancer

目的 探讨长链非编码RNA Linc00673在胃癌患者血清中的表达水平,评估其单项及与糖类抗原72-4(carbohydrate antigen 72-4,CA72-4)联合检测对胃癌临床诊断的效能和意义。方法 选取100例胃癌患者血清标本作为试验组,76健康体检者血清标本作为对照组。通过实时定量聚合酶链反应检测2组血清Linc00673的表达水平。用受试者工作特征曲线及危险分数分析法评估Linc00673对胃癌的诊断效能。通过化学发光免疫分析法检测CA72-4的表达。通过计算曲线下面积(area under the curve,AUC)、敏感度、特异度、阳性预测值(positive predictive value,PPV)以及阴性预测值(negative predictive value,NPV),评估单项及联合检测的临床应用价值。结果 对照组和试验组的血清Linc00673相对表达量分别为1.48±1.12和4.24±2.75,差异有统计学意义(P<0.001)。血清Linc00673表达水平与年龄、临床分期及远处转移等均无关(均P>0.05),但与淋巴结转移呈负相关(P<0.05)。Linc00673诊断胃癌的AUC、敏感度和NPV分别为0.86,83.00%和76.39%,而CA72-4分别为0.77,31.00%和47.73%。CA72-4与Linc00673联合检测可将诊断的敏感度和NPV分别提高至88.00%和79.66%。结论 Linc00673在胃癌血清中高表达,是胃癌临床诊断的潜在血清学标志物。

Objective To explore the expression level of long non-coding RNA Linc00673 in the serum of patients with gastric cancer,and evaluate the efficacy and significance of its single and combined detection with carbohydrate antigen 72-4(CA72-4) in the clinical diagnosis of gastric cancer.Methods Serum samples from 100 gastric cancer patients were selected as the treatment group,and the serum samples from 76 healthy volunteers were used as the control group.The expression levels of serum Linc00673 in two groups were detected by real-time quantitative polymerase chain reaction.The diagnostic efficacy of Linc00673 for gastric cancer was evaluated by receiver operating characteristic curve and risk score analysis method.The expression of CA72-4 was detected by electrochemiluminescence immunoassay.The area under the curve(AUC),sensitivity,specificity,positive predictive value(PPV) and negative predictive value(NPV) were calculated to evaluate the clinical application value of single and combined detection.Results The relative expression ofLinc00673 in the serum of control and treatment groups were 1.48 ± 1.12 and4.24 ± 2.75 with significant difference(P< 0.001).The serum expression level ofLinc00673 was not related to thefactors such as age,clinical stage and distant metastasis of the tumor(allP> 0.05),but was negatively related tolymph node metastasis of the tumor(P< 0.05).The AUC,sensitivity and NPV of Linc00673 in the diagnosis of gastriccancer were 0.86,83.00% and 76.39%,while CA72-4 were 0.77,31.00% and 47.73%,respectively.Thesensitivity and NPV of CA72-4 combined with Linc00673 increased to 88.00% and 79.66%,respectively.Conclusion Linc00673 is highly expressed in the serum of gastric cancer patients,which is a potential serologicalmarker for clinical diagnosis of gastric cancer.