绵羊清道夫受体MARCO基因启动子克隆及生物信息学分析

Cloning and Bioinformatics Analysis of Sheep Scavenger Receptor MARCO Gene Promoter

为了分析绵羊清道夫受体MARCO基因的启动子序列特点,初步研究其功能及转录调控机理,以绵羊肺组织DNA为模板,通过PCR扩增绵羊MARCO基因启动子序列,对其序列进行生物信息学分析,并构建双荧光素酶报告基因载体。结果显示,成功克隆绵羊MARCO基因启动子,大小为1002 bp;预测了其核心活性区域,发现有1个潜在的活性区域位于5′端-790~-741,同时发现了1个TATA-box元件和多个转录因子结合位点,未发现CpG岛。结果表明,位于绵羊MARCO基因启动子区域的TATA-box元件和Sp1、NF-1、NF-κB、C/EBPalp、c-Jun等转录因子,可能影响绵羊MARCO基因启动子的激活,为后续研究MARCO基因的转录调控机理提供了参考依据。

In order to analyze the promoter sequence structure of sheep scavenger receptor MARCO gene,preliminarily study its function and transcriptional regulation mechanism.The promoter sequence of sheep MARCO gene was amplified by PCR using sheep lung tissue DNA as the template.The sequence was analyzed by bioinformatics and the dual luciferase reporter gene vector was constructed.The results showed as follows:the sheep MARCO gene promoter was successfully cloned and the size was 1002 bp.The core active region was predicted and one potential active region was located at the 5′-741~-790.Besides,one TATA-box element and multiple transcription factor binding sites were found,no CpG island.These results suggested that TATA-box element located in the promoter region of MARCO gene and transcription factors such as Sp1,NF-1,NF-κB,C/EBPalp,c-Jun might play an important role in the expression of MARCO gene,which can provide reference for future research on the transcriptional regulation mechanism of MARCO gene.