Pita、Pib在宁夏及引进水稻种质资源中的分布及与穗颈瘟抗性的关系

Distribution of Pita and Pib Genes and Their Relationships with Neck Blast Resistance in Ningxia and Imported Rice Germplasm Resources

以85份宁夏回族自治区当地及引进水稻种质资源为试验材料,对其叶瘟和穗颈瘟抗性进行鉴定,分析Pita、Pib基因在这些水稻种质资源中的分布及其与穗颈瘟抗性的关系,明确这2个基因在水稻抗穗颈瘟方面的价值,为通过分子标记辅助选择培育抗穗颈瘟水稻新品种提供理论依据。结果表明,85份水稻种质资源中,抗叶瘟的有66份,抗病率为77.65%;抗穗颈瘟的有40份,抗病率为47.06%;综合抗稻瘟病的有56份,综合抗病率为65.88%,综合抗性评价最高能达到抗。85份水稻种质资源中,基因组合为Pita^(+)/Pib^(+)的有42份,对穗颈瘟的抗病率为40.47%,对稻瘟病的综合抗病率为69.05%;基因组合为Pita^(+)/Pib^(-)的有17份,对穗颈瘟的抗病率为70.59%,对稻瘟病的综合抗病率为82.35%;基因组合为Pita^(-)/Pib^(+)的有17份,对穗颈瘟的抗病率为47.06%,对稻瘟病的综合抗病率为52.94%;基因组合为Pita^(-)/Pib^(-)的有9份,对穗颈瘟的抗病率为33.33%,对稻瘟病的综合抗病率为44.44%。不同基因组合对穗颈瘟的抗性表现为Pita^(+)/Pib^(-)>Pita^(-)/Pib^(+)>Pita^(+)/Pib^(+)>Pita^(-)/Pib^(-),对稻瘟病的综合抗性表现为Pita^(+)/Pib^(-)>Pita^(+)/Pib^(+)>Pita^(-)/Pib^(+)>Pita^(-)/Pib^(-)。相关性分析结果表明,Pib基因与穗颈瘟抗性无显著的相关性,Pita基因与穗颈瘟抗性有显著的相关性。综上,Pita基因可以用于宁夏回族自治区水稻抗穗颈瘟育种,同时在生产上Pita基因也可以和Pib基因相结合,用于抗稻瘟病的基因聚合育种。

Eighty‑five Ningxia Hui Autonomous Region and imported rice germplasm resources were used as experimental materials to identify the resistance to leaf blast and neck blast.The distribution of Pita and Pib genes and their relationships with neck blast resistance were analyzed to clarify the value of these two genes in resistance to neck blast in rice germplasm resources in Ningxia Hui Autonomous Region,so as to provide theoretical basis for breeding new neck blast‑resistant rice varieties through molecular marker‑assisted selection.The results showed that 66 of 85 rice germplasm resources were resistant to leaf blast,with resistance rate of 77.65%,and 40 rice germplasm resources were resistant to neck blast,with resistance rate of 47.06%;56 rice germplasm resources were comprehensively resistant to rice blast,with resistance rate of 65.88%,and the highest level of comprehensive resistance evaluation reached resistance.Among 85 rice germplasm resources,there were 42 rice germplasm resources with Pita^(+)/Pib^(+)gene combination,the neck blast resistance rate was 40.47%,and the comprehensive rice blast resistance rate was 69.05%;there were 17 rice germplasm resources with Pita^(+)/Pib^(-)gene combination,the neck blast resistance rate was 70.59%,and the comprehensive rice blast resistance rate was 82.35%;there were 17 rice germplasm resources with Pita^(-)/Pib^(+)gene combination,the neck blast resistance rate was 47.06%,and the comprehensive rice blast resistance rate was 52.94%;there were 9 rice germplasm resources with Pita‑/Pib‑gene combination,the neck blast resistance rate was 33.33%,and the comprehensive rice blast resistance rate was 44.44%.The order of neck blast resistance of different gene combinations was Pita^(+)/Pib‑>Pita‑/Pib^(+)>Pita^(+)/Pib^(+)>Pita‑/Pib‑,and the order of comprehensive rice blast resistance was Pita^(+)/Pib‑>Pita^(+)/Pib^(+)>Pita‑/Pib^(+)>Pita‑/Pib‑.The correlation analysis results showed that there was no significant correlation between Pib gene and neck blast resistance,while Pita gene had significant correlation with neck blast resistance.Overall,Pita gene can be used in the breeding of rice resistance to neck blast in Ningxia Hui Autonomous Region,in the meantime Pita gene can be combined with Pib gene in the breeding of rice blast resistance.