伊犁马MCT1基因多态性与速度性能的关联性分析

Correlation Analysis between MCT1 Gene Polymorphismsand Speed Performance in Yili Horses

【目的】研究伊犁马MCT1基因多态性与速度性能的关联性,为专门化用途速度马的选种育种提供技术支撑。【方法】以50匹参加2000 m速度赛的2岁伊犁马为研究对象,运用DNA直接测序法筛选伊犁马MCT1基因第2、3外显子的突变位点,分析其多态性位点以及与伊犁马2000 m比赛成绩的关联性。【结果】在伊犁马MCT1基因第2外显子上发现一个SNP位点,在伊犁马MCT1基因第3外显子上没有发现突变位点,在g.52168582处,碱基T突变为碱基G,此突变位点为错义突变,该突变导致其编码氨基酸由苯丙氨酸变为亮氨酸,属于中度多态位点,处于Hardy-Weinberg平衡状态,共存在TT、TG、GG 3种基因型,TT基因平均速度高于GG和TG基因型平均速度,且TT基因型平均速度显著高于GG基因型平均速度(P<0.05)。【结论】在伊犁马MCT1基因第2外显子g.52168582T>G处TT基因型更有利于伊犁马2000 m比赛速度。

【Objective】The study the correlation between MCT1 gene polymorphism and speed performance in Yili horses,and provide technical support for the selection and breeding of specialized speed horses.【Methods】502-year-old Yili horses participating in the 2,000 m speed race were selected as the research objects.The mutation site of exon 2 and 3 of MCT1 gene of Yili horse was screened by DNA direct sequencing method,and analyze its polymorphism loci and its association with the performance of Yili horse 2000 m race.【Results】SNP site was found on the second exon of the Yili horse MCT1 gene,but no mutation site was found on the third exon of the Yili horse MCT1 gene.At g.52168582,the base T was mutated to the base G,and the mutation site was a missense mutation,which caused the encoded amino acid to be changed from phenylalanine.Acid changes to leucine,which belongs to moderate polymorphism site and is in a Hardy-Weinberg equilibrium state.There are three genotypes of TT,TG and GG.The correlation analysis with the 2000 m race results shows that the average speed of TT gene is higher than that of GG and GG.The average speed of TG genotype,and the average speed of TT genotype was significantly higher than that of GG genotype(P<0.05).【Conclusion】The TT genotype at the second exon g.52168582T>G of the MCT1 gene of Yili horses is more conducive to the 2000 m race speed of Yili horses.