MicroRNA-122-5P通过靶向MMP-9抑制TPA诱导的SW480细胞侵袭迁移能力

MicroRNA-122-5P Inhibits TPA-Induced Invasive Migration of SW480 Cells by Targeting MMP-9 to

目的:研究miR-122-5p对TPA诱导人结直肠癌SW480细胞MMP-9表达及侵袭迁移的影响。方法:利用生物信息学筛选结直肠癌差异基因,预测其靶基因miRNA。用TPA(100nM)处理SW480细胞,通过RT-qPCR、Western blot法检测基因和蛋白表达水平;明胶酶谱法检测MMP-9细胞外分泌情况;Transwell检测细胞侵袭和迁移能力。结果:MMP-9在结肠癌组织中高表达,筛选出上游靶基因miR-122-5P;TPA诱导MMP-9 mRNA表达呈时间依赖性上调(P<0.01),诱导miR-122-5p的表达下调(P<0.01);过表达miR-122-5p明显下调TPA诱导的MMP-9 mRNA和蛋白表达(P<0.05),且MMP-9胞外分泌减少,细胞的侵袭、迁移能力显著下降(P<0.01)。结论:miR-122-5p可能通过调控TPA诱导MMP-9的表达,进而抑制结直肠癌SW480细胞侵袭和迁移能力,为克服结直肠癌转移研究提供新的思路。

Objective:To study the effect of microRNA-122-5p on the expression,invasion and migration of MMP-9 in human colorectal cancer SW480 cells induced by TPA.Methods:Bioinformatics was used to screen colorectal cancer differential genes and predict their target miRNA.SW480 cells were treated with TPA(100 nM),and gene and protein expression levels were detected by RT-qPCR and Western blot;gelatin zymography was used to detect MMP-9 cell exocytosis;Transwell was used to detect cell invasion and migration ability.Results:MMP-9 was highly expressed in colon cancer tissues,and the upstream target gene miR-122-5P was screened;TPA induced a time-dependent upregulation of MMP-9 mRNA expression(P<0.01)and a downregulation of miR-122-5p expression(P<0.01);overexpression of miR-122-5p significantly downregulated TPA-induced MMP-9 mRNA and protein expression(P<0.05),the extracellular secretion of MMP-9 was reduced,and the invasion and migration ability of cells were significantly decreased(P<0.01).Conclusion:miR-122-5p may induce MMP-9 expression by regulating TPA and thus inhibit the invasive and migratory ability of colorectal cancer SW480 cells,providing a new idea to overcome the metastasis study of colorectal cancer.