泛癌中SMYD3的表达与预后及免疫浸润之间的关系

Relationships between progrosis,immune infiltration and expression of SMYD3 in pan-cancer

目的研究SMYD3表达在泛癌中的预后价值,分析SMYD3对于肿瘤组织免疫浸润的影响。方法从TCGA、GTEx、HPA等数据库收集基因表达矩阵以及蛋白质表达等数据。使用Kaplan-Meier算法评价了SMYD3在不同肿瘤中的预后价值。使用ESTIMATE算法和Timer数据库的信息分析SMYD3与免疫浸润的关系。以小鼠黑色素瘤细胞系B16为例,在细胞水平验证SMYD3对抗原提呈基因表达的影响。结果转录组和蛋白组学数据表明SMYD3在肿瘤组织中表达升高,并且SMYD3的高表达和泛癌的不良预后相关(P<0.05);SMYD3抑制了多种免疫细胞的浸润(P<0.05),敲除Smyd3的B16细胞抗原呈递基因表达水平升高(P<0.05)。结论Smyd3是一个重要的肿瘤预后相关基因,其通过下调抗原递呈基因的表达,抑制免疫细胞浸润,诱导肿瘤细胞免疫逃逸。

Objective The study focuses on the prognostic value of SMYD3 in pan cancer,and analyze the influence of SMYD3 on tumor tissue immune infiltration.Methods The gene expression and protein density data were collected from TCGA,GTEx and HPA databases.Kaplan-Meier(KM)algorithm was used to evaluate the impact of SMYD3 on the prognosis of patients with different kinds of tumor.The ESTIMATE algorithm and Timer database were applied to investigate the relationship between SMYD3 and immune microenvironment.And the results were verified in B16 cell line by knockout SMYD3.Results Higher expression of SMYD3 was observed in tumor tissue than normal tissue in both mRNA and protein level.And SMYD3 was thought to be associated with worse outcomes of patients(P<0.05)and less immune cells inflation in tumor tissue(P<0.05).Knockout Smyd3 in B16 cell line increased the expression of antigen present genes(P<0.05).Conclusions Smyd3 is an important gene related to the prognosis of patients and the antigen presentation function of tumor cells.Smyd3 can inhibit the infiltration of immune cell by down-regulation of antigen-present genes,which induces the immune escape.