饥饿对日本囊对虾免疫及ATP酶活性的影响

Effects of Starvation Stress on Immunity and ATPase Activity of Kuruma Prawn Marsupenaeus japonicus

为探究饥饿胁迫对日本囊对虾生长、免疫和能量代谢的影响,在25℃条件下将体质量(0.8±0.06)g的日本囊对虾置于多层多格的格栅式养殖装置中,每格放置1尾个体进行0、3、6、9、12 d的饥饿胁迫,分别检测各时间点胃、肝胰腺和肌肉组织中各种指标的变化情况。试验结果表明:饥饿胁迫下,日本囊对虾生长性能显著降低;各组织中过氧化氢酶活性均呈现先升后降的趋势;酸性磷酸酶、碱性磷酸酶活性在各组织中均随饥饿时间的延长呈显著下降的趋势(P<0.05);超氧化物歧化酶的活性变化呈现出明显的组织特异性,胃组织中的超氧化物歧化酶活性在饥饿胁迫前期呈现上升趋势,并在饥饿3 d达到最高值,随着饥饿时间的延长显著下降,肝胰腺和肌肉组织中的超氧化物歧化酶活性则随着饥饿时间的延长呈显著下降的趋势;饥饿胁迫下日本囊对虾各组织中钠钾ATP酶和钙镁ATP酶活性均呈现先升后降的趋势,且均在胁迫的3 d达到最高值。本试验结果表明,饥饿胁迫不仅会显著降低日本囊对虾的生长性能,在不同胁迫时期对其各组织中免疫相关酶及ATP酶活性也有显著影响。

In this study,kuruma prawn Marsupenaeus japonicus with body weight of(0.8±0.06)g were placed in multi-layer and multi-cell grid culture system at 25℃,and one individual was placed in each cell for 0,3,6,9 and 12 days of starvation stress.The changes in various indices in stomach,hepatopancreas and muscle tissues were detected at each time point.The results showed that the starved kuruma prawn had the significantly poor growth performance;catalase(CAT)activities in all tissues were found to be increased first and then decreased under starvation;the activities of acid phosphatase(ACP)and alkaline phosphatase(AKP)were found to be decreased significantly with the prolongation of starvation time in all tissues(P<0.05).The activity of superoxide dismutase(SOD)showed obvious tissue specificity.The activity of SOD in gastric tissue was increased first and then decreased with the extension of starvation time,with the peak value on the third day of starvation.The activity of SOD in hepatopancreas and muscle tissue decreased continuously with the extension of starvation time.With the extension of stress time,the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in various tissues were found to be increased first and then decreased,with the maximal value on the third day of the stress.The findings showed that starvation stress not only significantly reduced the growth performance,but also significantly affected the activities of immune related enzymes and ATPase in different stress periods.