9种海洋贝类硫酸化多糖的提取、分离纯化及化学性质分析

Extraction, purification and chemical property analysis of sulfated polysaccharides from nine marine shellfishes

硫酸化多糖是贝类中重要的营养功效成分。本研究建立了一种适合海洋贝类动物硫酸化多糖微量快速提取及分离纯化的方法,并应用于9种贝类;测定硫酸化多糖的基本化学组成、分子量和单糖组成,并基于此初步判定其所含的糖胺聚糖(glycosaminoglycans, GAGs)种类。结果表明,所建立的方法能够实现多糖的快速分离纯化,纯化后9种贝类的多糖均为单一组分,分子量为24~55 kDa。纯化多糖的单糖组成及红外光谱结果表明,9种贝类中的硫酸化多糖具有类糖胺聚糖特性:蛾螺科和壳菜蛤科多糖样品呈现硫酸软骨素(chondroitin sulfate, CS)的特征,分别以水泡蛾螺和翡翠贻贝为代表;海湾扇贝多糖样品呈现肝素(heparin, HP)的特征。本研究为食用贝类硫酸化多糖的结构解析提供了微量快速的前处理技术。

Sulfated polysaccharide is an important nutritional component in shellfishes. In this study, a rapid microextraction and purification process for sulfated polysaccharides from marine shellfishes was established and applied to nine different shellfishes. Types of glycosaminoglycans(GAGs) from nine species of edible shellfishes were qualitatively analyzed by their basic chemical components, molecular weights and monosaccharide compositions. The results showed that the method could rapidly separate and purify polysaccharides. The purified polysaccharides were single components with molecular weights ranging from 24 to 55 kDa. Analysis of monosaccharide composition and infra-red spectra results showed that sulfated polysaccharides had GAGs-like properties. The polysaccharide samples from Buccinidae and Trichomya exhibited the characteristics of chondroitin sulfate(CS), which were represented by Buccinum pemphigum and Perna viridis. The polysaccharide samples from Argopecten irradians showed heparin(HP) characteristics. This study provides a micro-and rapid pretreatment technology for the structural analysis of sulfated polysaccharides from edible shellfishes.