2019—2021年北京市种猪场猪伪狂犬病病毒感染情况调查

Investigation on Pseudorabies Virus Infection in Swine Breeding Farms in Beijing City from 2019 to 2021

为了解北京市种猪场猪群伪狂犬病病毒(PRV)感染情况,采用酶联免疫吸附试验(ELISA),对2019—2021年3个区采集的10850份血清样品进行PRV感染抗体(gE抗体)检测。结果显示:2019—2021年,北京市3个区种猪场群体表观阳性率分别为84.38%、77.78%、64.71%,群体真实阳性率分别为86.10%(95%CI:73.95%~98.24%)、79.37%(95%CI:60.39%~98.34%)、66.03%(95%CI:43.22%~88.83%),下降趋势不显著(P>0.05);个体表观阳性率分别为53.95%、41.52%、35.59%,个体真实阳性率分别为55.05%(95%CI:53.05%~57.05%)、42.37%(95%CI:40.90%~43.84%)、36.32%(95%CI:34.85%~37.79%),呈显著下降趋势(P<0.05)。育成猪、经产母猪和后备母猪个体阳性率逐年下降,而育肥猪个体阳性率逐年升高,由2019年的38.45%,提高到2021年的60.79%。结果表明,北京市种猪场PRV感染得到一定的控制,但感染情况依然严重,尤其是育肥猪,因此应继续加强免疫和监测及生物安全体系建设,逐步实现种猪场净化和根除PR的目标。

In order to investigate the infection with pseudorabies virus(PRV)in swine breeding farms in Beijing City,10850 serum samples collected from 3 districts from 2019 to 2021 were detected for antibodies against PRV(gE antibodies)by enzyme-linked immunosorbent assay(ELISA).The results showed that the population apparent positive rates of the farms for three consecutive years were 84.38%,77.78%and 64.71%respectively,and the true ones were 86.10%(from 73.95%to 98.24%),79.37%(from 60.39%to 98.34%)and 66.03%(from 43.22%to 88.83%),respectively,with a slightly downtrend(P>0.05);the individual apparent positive rates were 53.95%,41.52%and 35.59%,respectively,and the true ones were 55.05%(from 53.05%to 57.05%),42.37%(from 40.90%to 43.84%)and 36.32%(from 34.85%to 37.79%)respectively,with a significant downtrend(P<0.05).The individual positive rates of growers,multiparous sows and reserve sows decreased year by year,while that of fattening pigs increased from 38.45%in 2019 to 60.79%in 2021.In conclusion,PRV infection was controlled in the breeding farms to some extent,although still serious,especially in fattening pigs,which should continue to strengthen immunization,monitoring and construction of a biosafety system,and to gradually achieve the goal of purification and eradication of PRV in farms.