糙米发酵滤液——一种具有抗衰老潜力的物质

Fermented brown rice filtrate:a substance with anti-aging potential

本研究以糙米水为底物,通过微生物发酵技术制备了糙米发酵滤液。通过对比发酵前后滤液,发现发酵可以增加可溶性固形物以及小分子物质含量。通过人类永生化表皮细胞(HaCaT)活性实验及DPPH自由基清除实验对发酵前后滤液进行功效比较。发酵前糙米滤液只有较弱的DPPH自由基清除能力,而糙米发酵滤液具有较强的DPPH自由基清除能力,并能提高HaCaT细胞活性。进一步研究发现,糙米发酵滤液能够减少细胞内ROS生成、提高细胞自噬标记物LC3B表达、以及减少糖化导致的Ⅰ型胶原蛋白流失。当作用于HaCaT细胞时能够促进透明质酸(HA)的生成和其受体CD44的表达。当作用于人原代成纤维细胞(FB)时能够促进胶原蛋白的生成。本研究表明,糙米发酵滤液具有多方面抗衰老的潜在能力。

In recent years,brown rice has gained growing attention due to its obvious advantage of being rich in bioactive compounds.The objective of this study was to evaluate the anti-aging potential of fermented brown rice filtrate(FBR) by Saccharomyces cerevisiae for cosmetics.Results for the FBR were compared to the brown rice aqueous solution(BR) that was not fermented.In this study,the total matter content of brown rice is almost unchanged before and after fermentation,but the small molecular matter increases obviously.In a DPPH free radical scavenging test,the antioxidant activity is greater in FRG compared to RG.In a human epidermal keratinocyte(HaCaT) proliferation test,FBR with a volume fraction of 0.2% and 1% shows the high proliferative capacity,while the BR with a volume fraction of 1% is characterized by high cytotoxicity to HaCaT.Meanwhile FBR shows anti-aging activity by increasing the expression of autophagy marker LC3B and Collagen I after AGEs-treated,while decreasing intracellular ROS production.Further study shows that FBR can promote the production of hyaluronic acid(HA) and the expression of its receptor CD44 in HaCaT,and enhance the expression of collagen I in human primary fibroblasts(FB).Taken together,these data indicates that FBR has the potential of anti-aging may be applied to cosmetics products.