意大利蜜蜂乙醇胺激酶1蛋白的结构域、空间结构和互作蛋白分析

Domains,Spatial Structures and Interaction Proteins of Apis mellifera ligustica Ethanolamine Kinase 1

蜜蜂是优秀的传粉者,它能够为多种农作物和野生植物授粉,在生态系统服务中扮演关键角色。然而,蜜蜂在觅食、繁殖过程中常遭受到热、农药、病虫害等胁迫因子的影响,其种群数量已呈现出锐减趋势。为了缓解这一问题,我们对意大利蜜蜂体内的抗逆因子进行了筛选,发现乙醇胺激酶1(Ethanolamine kinase 1,ETNK1)受到阿维菌素的诱导表达。旨在利用生物信息学工具,对意大利蜜蜂ETNK1的结构域、空间结构和互作蛋白网络进行构建和分析。结果显示,ETNK1具有保守的ETNK-euk结构域,属于PK-like超家族。其二级结构包含15个α螺旋、9个β折叠和多个无规则卷曲。根据蛋白质互作分析结果,ETNK1与乙醇胺磷酸胞苷酰转移酶和磷酸胆碱胞苷酰转移酶A可能有互作关系。有望为培养恶劣环境下高抗逆性蜂种提供思路。

Honeybees are excellent pollinators,which can pollinate a variety of crops and wild plants and play a key role in ecosystem services.However,honeybees are often affected by heat,pesticides,diseases and insect pests and other stress factors in the process of foraging and reproduction,and their population has shown a sharp decline trend.In order to alleviate this problem,we screened the stress-resistant factors in Italian honeybee and found that ethanolamine kinase 1(Ethanolamine kinase1,ETNK1)was induced by avermectin.The purpose of this paper is to construct and analyze the domains,spatial structures and interaction proteins network of Apis mellifera ligustica ETNK1 by bioinformatics tools.The results show that ETNK1 has a conservative ETNK_euk domain and belongs to the PK-like superfamily.Its secondary structure consists of 15α-helixes,9β-folds and multiple irregular crimps.According to the results of protein interaction analysis,ETNK1 may interact with ethanolamine phosphate cytidyl transferase and phosphocholine cytidyl transferase A.This study is expected to provide ideas for the cultivation of highly stress-resistant bee species in harsh environments.