宏基因组学测序技术在成人医院获得性肺炎中的临床应用专家共识

Expert consensus on the clinical application of metagenomic sequencing technology in adult hospital-acquired pneumonia

医院获得性肺炎主要是指包括细菌、真菌、病毒、原虫及支/衣原体等病原体在入院48 h后引发的各类肺实质炎症。对于不明病原体感染的医院获得性肺炎,宏基因组测序技术能够检测几乎所有病原体核酸,所以该技术尤为适用于病原检测。宏基因测序技术实验流程操作略显繁琐,分析流程复杂,检测结果冗长,因此对实验室操作人员及解读人员的素质要求颇高,对相关的质量控制也需要标准化。简述了该技术在医院获得性肺炎中的应用及关键环节的质量控制。

Hospital-acquired pneumonia is an lung parenchymal inflammation mainly caused by pathogens including bacteria,fungi,viruses,protozoa and Mycoplasma/Chlamydia after 48 hours to admission.For hospital-acquired pneumonia infected by unknown pathogens,mNGS technology can detect almost all pathogen nucleic acids,so this technique is particularly suitable for pathogen test.mNGS technology experiment operation is cumbersome,the analysis process is complex,and the test results are lengthy,so the quality requirements of laboratory operators and interpreters are quite high,and the relevant quality control also needs to be standardized.have been briefly described the application of this technology in hospital-acquired pneumonia and the quality control of key links.