不同运动方式对人体DNA损伤、DNA甲基化和端粒长度的影响

Effects of different exercise types on human DNA damage,DNA methylation and telomere length

背景:运动不仅是改善身体健康和心理健康的有效手段,还对代谢性和心脑血管等疾病的发生、发展具有良好的干预效果,其原因与表观遗传因素有关。目的:总结不同运动方式对人体DNA损伤、DNA甲基化和端粒长度的影响,并分析运动调控表观遗传修饰的可能机制,以期为运动改善机体功能提供参考。方法:以“运动,有氧训练,急性运动,无氧训练,抗阻训练,DNA损伤,DNA甲基化,端粒”为中文检索词,以“exercise,sport,aerobic exercise,anaerobic exercise,resistance training,acute exercise,DNA methylation,DNA damage,telomere”为英文检索词。在PubMed、Embase、Web of Science、中国知网数据库中进行检索,并根据纳入与排除标准筛选文献,最终纳入70篇文献。结果与结论:①长期有氧、抗阻和无氧运动均能改善DNA损伤,其原因与运动可以提高机体的抗氧化能力有关。而急性运动则会通过上调活性氧和活性氮氧化物的表达进而加剧DNA损伤程度;②急性运动、长期抗阻运动和无氧运动在降低DNA甲基化方面具有积极作用,其关键机制可能是运动诱导的活性氧使氧化型谷胱甘肽/还原型谷胱甘肽比值和DNA甲基化转移酶、10-11易位酶的表达发生了改变,进而对DNA甲基化产生调控作用;③与其他运动形式相比,长时间有氧运动可能更具有增加端粒长度的潜在价值,其中的生物学机制涉及炎症、氧化应激、DNA甲基化和微小RNA的表达调控;④基于当前文献可知,有氧运动持续2年以上可以增加端粒长度,未来的研究也应进一步明确最佳的运动持续时间。

BACKGROUND:Exercise is not only an effective means to improve physical and mental health,but also has a good intervention effect on the occurrence and development of metabolic,cardiovascular and cerebrovascular diseases.The reason is related to the epigenetic factors.OBJECTIVE:To summarize the effects of different exercise types on human DNA damage,DNA methylation,and telomere length,and analyze the possible mechanism of exercise regulation epigenetic modification,in order to provide a reference for exercise to improve body function.METHODS:“Exercise,aerobic training,acute exercise,anaerobic training,resistance training,DNA damage,DNA methylation,telomere”were used as the Chinese search terms,and“exercise,sport,aerobic exercise,anaerobic exercise,resistance training,acute exercise,DNA metabolism,DNA damage,telomere”were used as the English search terms.We searched PubMed,Embase,Web of Science,and CNKI databases,and screened articles according to inclusion and exclusion criteria,and finally included 70 articles.RESULTS AND CONCLUSION:(1)Long-term aerobic,resistance,and anaerobic exercises can improve DNA damage.The reason is that exercise can improve the body’s antioxidant capacity.Acute exercise can aggravate the degree of DNA damage by up-regulating the expression of reactive oxygen species and reactive nitrogen oxides.(2)Acute exercise,long-term resistance exercise,and anaerobic exercise play a positive role in reducing DNA methylation.The key mechanism may be that exercise-induced reactive oxygen species changes the expression of glutathione oxidized/glutathione,DNA methyltransferase,and 10-11 translocation enzyme.Then it can regulate DNA methylation.(3)Compared with other types of exercise,long-term aerobic exercise may have more potential value in increasing telomere length,and its biological mechanism involves inflammation,oxidative stress,DNA methylation,and regulation of microRNAs(miRNAs)expression.(4)Based on the current literature,aerobic exercise lasting at least 2 years can increase telomere length,and future research should further clarify the optimal exercise duration.