高效液相色谱法测定茶干中山梨酸含量及其不确定度评定

Determination of Sorbic Acid in Dried Bean Curd by High Performance Liquid Chromatography and Its Uncertainty Evaluation

根据《测量不确定度评定与表示》(JJF 1059.1—2012)和《化学分析中不确定度的评估指南》(CNAS—GL 006:2019)的规定,对高效液相色谱法测定茶干中山梨酸含量的不确定度进行分析,考察不确定度主要来源,并计算合成不确定度和扩展不确定度,为高效液相色谱法测定茶干中山梨酸含量的不确定度评价提供参考。结果表明,山梨酸检测浓度为0.675 g·kg^(-1),其相对不确定度为0.0122,扩展不确定为0.0165 g·kg^(-1)。该方法测定茶干中山梨酸含量的不确定度主要来源于玻璃量具、标准物质以及标准曲线拟合。

According to the provisions of JJF 1059.1—2012 and CNAS—GL 006:2019,the uncertainty of the method of determination of sorbic acid in dried bean curd by high performance liquid chromatography was analyzed,the main sources of uncertainty were investigated,and the synthetic uncertainty and extended uncertainty were calculated,providing a reference for uncertainty evaluation in the determination of sorbic acid in dried bean curd by high performance liquid chromatography.The results showed that the detection concentration of sorbic acid was 0.675 g·kg^(-1),the relative uncertainty was 0.0122,and the expanded uncertainty was 0.0165 g·kg^(-1).The uncertainty in the determination of sorbic acid content in dried tea by this method mainly comes from glass measuring tools,standard materials,and standard curve fitting.