附仙强心方对慢性心力衰竭阳虚水泛证大鼠sST2、CaN的影响

Effect of Fuxian Qiangxin Recipe on sST2,CaN in chronic heart failure rats with yang deficiency and water flooding syndrome

目的观察附仙强心方对阿霉素诱导的慢性心力衰竭(CHF)大鼠心肌重构相关指标的影响,探究附仙强心方对CHF的干预作用。方法60只SPF级雄性Wistar大鼠随机分为6组,分别为正常组、模型组、卡托普利组,以及附仙强心方低、中、高剂量组,每组10只。除正常组外,其余各组应用腹腔注射阿霉素盐酸盐复制心力衰竭模型,然后分别给予相应的干预措施。6周后观察各组大鼠一般情况,应用苏木精-伊红(HE)染色法比较心肌组织形态学情况,心脏彩超检测心功能情况,酶联免疫吸附试验(ELISA)检测血清可溶性基质裂解素2(sST2)含量,蛋白免疫印迹(Western blot)法检测心肌组织钙调神经磷酸酶(CaN)蛋白表达水平。结果①一般情况:与正常组比较,模型组大鼠出现体质量明显减轻、活动少、被毛无光泽、口鼻暗淡、小便量多、大便稀薄等阳虚症状;各治疗组症状较模型组改善。②病理观察:模型组较正常组心肌细胞核深染,可见局部空泡化,提示心肌细胞损伤;附仙强心方各剂量组与模型组比较,心肌细胞损伤有不同程度的减轻与改善。③心功能:与正常组比较,模型组左心室射血分数(LVEF)降低,收缩末期内径(LVIDs)、舒张末期内径(LVIDd)均显著升高(P<0.05);与模型组比较,附仙强心方各剂量组LVEF均显著升高,LVIDs均显著降低(P<0.05)。④sST2、CaN检测:与正常组比较,模型组血清sST2、心肌组织CaN水平均显著升高(P<0.05);与模型组比较,附仙强心方各剂量组sST2、CaN水平均降低(P<0.05)。结论附仙强心方可保护CHF大鼠心肌,有效改善心功能,延缓心肌重构,其机制可能与抑制血清sST2及心肌组织CaN的表达有关。

Objective To observe the effect of Fuxian Qiangxin Recipe on related indexes of myocardial remodeling in rats with chronic heart failure(CHF)induced by adriamycin,and to explore the intervention effect of Fuxian Qiangxin Recipe on heart failure.Methods Sixty SPF male Wistar rats were randomly divided into 6 groups:normal group,model group,captopril group,and Fuxian Qiangxin Recipe low,medium and high dose group,with 10 rats in each group.Except for the normal group,other groups were treated with intraperitoneal injection of adriamycin to replicate chronic heart failure models,and then given corresponding intervention measures.After 6 weeks,the general conditions of the rats in each group were observed.The morphological changes of myocardium were compared by HE staining.The cardiac function of the rats in each group was detected by cardiac color Doppler ultrasound.Enzyme-linked immunosorbent assay(ELISA)was used to detect the content of soluble matrix lysin-2(sST2)in rat serums,and Western blot was used to detect the protein expression of calcineurin(CaN)in rat myocardial tissues.Results①General condition:Compared with the normal group,the rats in the model group showed symptoms of yang deficiency,such as obvious weight loss,less activity,dull hair,dim nose and mouth,excessive urination and thin stool.The symptoms of each treatment groups were improved compared with those of the model group.②Pathological observation:The myocardial nucleus of the model group was more stained than that of the normal group,showing local vacuolation,indicating cardiomyocyte injury;compared with the model group,the degree of cardiomyocyte injury in each dose group of Fuxian Qiangxin Recipe was alleviated and improved in varying degrees.③Cardiac function:compared with the normal group,the left ventricular ejection fraction(LVEF)decreased,the end-systolic diameter(LVIDs)and end-diastolic diameter(LVIDd)increased significantly in the model group(P<0.05),and the LVEF increased significantly and LVIDs decreased significantly in each group of Fuxian Qiangxin Recipe compared with the model group(P<0.05).④Detection of sST2 and CaN:Compared with the normal group,the levels of serum sST2 and myocardial tissue CaN in the model group were significantly higher than those in the normal group,while the levels of sST2 and CaN in each dose group of Fuxian Qiangxin Recipe were lower than those in the model group(P<0.05).Conclusion Fuxian Qiangxin Recipe can protect myocardium,improve cardiac function and delay myocardial remodeling in rats with heart failure.The mechanism may be related to the inhibition of serum sST2 and myocardial tissue CaN expression.