YAP、p-STAT3、LMP2A在鼻咽癌中的表达及临床意义

Expression and clinical significance of YAP,p-STAT3,LMP2A in nasopharyngeal carcinoma

目的探讨Yes相关蛋白(YAP)、磷酸化信号转导与转录激活因子3(p-STAT3)、外源性表达潜伏膜蛋白2A(LMP2A)在鼻咽癌(NPC)中的表达及临床意义。方法收集2020年12月至2021年12月于临沂市人民医院接受治疗的202例NPC患者的活检术石蜡标本作为观察组,同时收集本院98例鼻咽黏膜慢性炎组织标本作为对照组。分析两组YAP、p-STAT3及LMP2A的表达,并分析YAP、p-STAT3及LMP2A三指标与NPC患者的临床病理参数、预后关系。结果观察组YAP、p-STAT3、LMP2A蛋白高表达率均高于对照组,差异有统计学意义(P<0.05)。不同TNM分期、组织分化以及有无远处转移NPC患者之间YAP表达比较差异有统计学意义(P<0.05);不同TNM分期和有无淋巴结、远处转移NPC患者之间p-STAT3表达比较差异有统计学意义(P<0.05);不同TNM分期、组织分化以及有无淋巴结转移NPC患者之间LMP2A表达比较差异有统计学意义(P<0.05)。经随访后发现,预后良好组181例,预后不良组21例;两组年龄、性别比较差异无统计学意义(P>0.05)。不同预后患者TNM分期、淋巴结及远处转移情况、YAP、p-STAT3、LMP2A高表达率比较差异均有统计学意义(P<0.05)。经Logistic回归分析可知,TNM分期为Ⅲ+Ⅳ期、有淋巴结及远处转移、YAP、p-STAT3及LMP2A高表达是影响NPC患者预后不良的独立危险因素(P<0.05)。结论NPC患者中YAP、p-STAT3、LMP2A呈上升表达,且三指标高表达与NPC病情的发生发展以及预后之间存在密切联系,对预测患者预后不良具有重要意义。

Objective To investigate the expression and clinical significance of Yes Associated Protein(YAP),phosphorylated signal transducers and activators of transcription(p-STAT3),latent membrane protein 2A(LMP2A)in nasopharyngeal carcinoma(NPC).Methods The biopsy paraffin specimens of 202 NPC patients treated in Linyi People's Hospital from December 2020 to December 2021 were retrospectively collected as the observation group,at the same time,98 tissue samples of chronic nasopharyngeal mucosal inflammation in our hospital were collected as the control group.The expression of YAP,p-STAT3 and LMP2A in the two groups was analyzed,and the relationship between the three indicators of YAP,p-STAT3 and LMP2A and the clinicopathology and prognosis of NPC patients was analyzed.Results The high expression rates of YAP,p-STAT3,and LMP2A proteins in the observation group were higher than those in the control group,and the difference was statistically significant(P<0.05).There were statistically significant differences in the expression of YAP between NPC patients with different TNM stages,tissue differentiation,and presence or absence of distant metastasis(P<0.05).There were statistically significant differences in the expression of p-STAT3 between NPC patients with different TNM stages,presence or absence of lymph nodes,and distant metastasis(P<0.05).There were statistically significant differences in the expression of LMP2A among NPC patients with different TNM stages,tissue differentiation and lymph node metastasis(P<0.05).After follow up,it was found that there were 181 cases in the good prognosis group and 21 cases in the poor prognosis group.There was no significant difference in age and gender between the two groups(P>0.05).There were statistically significant differences in TNM staging,lymph node metastasis and distant metastasis,as well as high expression rates of YAP,p-STAT3,and LMP2A among patients with different prognosis(P<0.05).Logistic regression analysis showed that the presence or absence of different stages,lymph node metastasis,and distant metastasis in TNM,YAP,p-STAT3 and LMP2A were independent risk factors affecting the poor prognosis of NPC patients(P<0.05).Conclusion The expressions of YAP,p-STAT3,and LMP2A are all elevated in NPC patients,and there is a close relationship between the high expression of the three indicators and the occurrence,development,and prognosis of NPC,which is of great significance for predicting poor prognosis.