基于pegB基因的鸡白痢沙门氏菌、鸡伤寒沙门氏菌分子鉴别方法的建立

pegB-PCR for Differentiation of Salmonella Gallinarum Biovar Pullorum and Gallinarum

为建立一种能快速、准确、简便区分鸡白痢沙门氏菌和鸡伤寒沙门氏菌的方法,研究通过对鸡白痢沙门氏菌和鸡伤寒沙门氏菌pegB序列进行分析,发现存在3个单核苷酸多态性位点,基于此建立了一种特异性PCR方法用于鉴别鸡白痢沙门氏菌和鸡伤寒沙门氏菌。结果显示,该方法特异性强、耗时短,检测限为10.14 pg/反应。临床样品检测表明,该方法不仅具有良好的特异性,且操作比传统方法更为简便。该方法的建立为准确区分鸡白痢沙门氏菌和鸡伤寒沙门氏菌提供了实用技术,将在分子检测中发挥重要作用。

The objective of the present study was to establish a rapid,accurate and simple method for differentiation of Sal⁃monella Pullorum and Salmonella Gallinarum.In this study,three single nucleotide polymorphism loci were found by analyzing the pegB sequence of Salmonella Pullorum and Salmonella Gallinarum.Based on this,a specific PCR method was developed for the identification of these two biovars.The results showed that the method was rapid,simple,specific,and the detection limit was 10.14 pg/reaction.Clinical sample testing showed that this method not only had good specificity,but also was more convenient to operate than traditional methods.The establishment of this method could provide a practical technique for differentiation of Sal⁃monella Pullorum and Salmonella Gallinarum,and would play an important role in molecular detection.