尿核基质蛋白22和非典型细胞参数在膀胱癌诊断中的临床价值

Clinical roles of nuclear matrix protein 22 and Atyp.C parameter in diagnosis of bladder cancer

目的探讨尿核基质蛋白22(NMP22)和尿有形成分分析仪非典型细胞(Atyp.C)参数联合检测诊断膀胱癌的价值。方法选取2021年10月—2022年9月上海市静安区市北医院行NMP22检测和尿有形成分分析的患者3288例,其中膀胱癌患者97例(膀胱癌组,均为男性)、其他泌尿系统疾病患者3191例[总对照组,其中男1212例、女1979例];将1212例男性泌尿系统疾病患者作为男性对照组。采用受试者工作特征(ROC)曲线评价各项指标诊断膀胱癌的效能。结果膀胱癌组NMP22阳性率和Atyp.C荧光强度显著高于男性对照组和总对照组(P<0.001)。ROC曲线分析结果显示,以男性对照组为参考,NMP22、Atyp.C荧光强度单项检测和联合检测诊断膀胱癌的曲线下面积(AUC)分别为0.794、0.757、0.867。以总对照组为参考,NMP22、Atyp.C荧光强度单项检测和联合检测诊断膀胱癌的AUC分别为0.787、0.752、0.861。结论在膀胱癌的诊断中,NMP22具有较高的敏感性,Atyp.C参数具有较高的特异性,联合检测可显著提高诊断效能。

Objective To investigate the clinical role of the combined determination of nuclear matrix protein 22(NMP22)and atypical cells(Atyp.C)parameter using urinary sediment analyzer in the diagnosis of bladder cancer.Methods Totally,3288 patients with NMP22 and urinary sediment determinations in Jing'an District Shibei Hospital were enrolled from October 2021 to September 2022.There were 97 patients with bladder cancer(bladder cancer group,97 males)and 3191 patients with other urinary system diseases(total control group,1212 males and 1979 females).A total of 1212 male patients with urinary system diseases were used as male control group.Receiver operating characteristic(ROC)curve was used to evaluate the efficiency of each index in the diagnosis of bladder cancer.Results The positive rate of NMP22 and thefluorescence intensity of Atyp.C in bladder cancer group were higher than those in male control group and total control group(P<0.001).ROC curve analysis results showed that the areas under curves(AUC)of NMP22 and Atyp.C fluorescence intensity single and combined determinations in the diagnosis of bladder cancer were 0.794,0.757 and 0.867,respectively,when the male control group was taken as control group.When the total control group was used as control group,the AUC of NMP22 and Atyp.Cfluorescence intensity single and combined determinations were 0.787,0.752 and 0.861,respectively.Conclusions In the diagnosis of bladder cancer,NMP22 has a high sensitivity,Atyp.C has a high specificity,and the combined determination can improve the diagnostic efficiency.