猫尾草叶绿体基因组结构及其解析

Structure of Uraria crinita Chloroplast Genome

【目的】阐明岭南药食两用植物猫尾草的叶绿体基因组结构特征,为猫尾草资源的保护、利用和开发提供理论依据。【方法】采用高通量测序技术开展猫尾草叶绿体基因组测序,并通过生物信息方法进行拼接、注释、解析以及系统进化分析。【结果】猫尾草叶绿体基因组是149774 bp的环状双链四段式分子,包含128个基因,GC含量为38.2%。猫尾草叶绿体基因组含有26015个密码子,偏好以A或T结尾;存在110个简单重复序列,以A或T单核苷酸重复居多。序列比对和进化分析显示猫尾草与同属狸尾豆的亲缘关系最近。【结论】首次报道猫尾草叶绿体基因组的全序列,并明确其结构特点,为猫尾草的栽培育种、遗传多样性和资源利用等奠定基础。

【Objective】Structure of the chloroplast genome from Uraria crinita,a plant used as food as well as herbal medicine in Lingnan area of China,was studied.【Methods】The whole chloroplast genome was obtained by high-throughput sequencing and then assembled,annotated,and analyzed by bioinformatic means.【Results】The genome was an annular quadripartite molecule of 149774 bp that harbored 128 genes.It had a low GC content of 38.2%containing 26015 codons that mostly ended with A or T.There were 110 loci of simple sequence repeat detected with the mononucleotide largely formed by A or T.An alignment and phylogenetic analysis on the sequence indicated a close relationship between U.crinita and U.lagopodioides.【Conclusion】The chloroplast genome and structure of U.crinita were unveiled for the first time to aid future studies on the cultivation,breeding,genetics,and utilization of the valuable plant.