枳椇子、葛花对小鼠酒精性肝损伤及胃溃疡的保护作用研究

Protective Effect Study of Hovenia Dulcis and Pueraria Lobata on Alcoholic Liver Injury and Gastric Ulcer of Mice

目的 探究葛花和枳椇子提取物对小鼠急性酒精性肝损伤及胃溃疡的保护作用。方法 将60只小鼠适应性饲养,1周后随机分为5组(n=12),分别为模型组、空白组、葛花组、枳椇组、枳葛组。除空白组外,其余各组小鼠每日给予相应干预药物4h后用50%乙醇10g/kg灌胃,连续14d,末次乙醇灌胃6h后,将小鼠麻醉,取血清分别进行谷丙转氨酶(ALT)、谷草转氨酶(AST)活性检测,另取肝、胃组织进行病理组织学观察。结果 各给药组血清中ALT和AST均显著低于模型组(P<0.01),略高于空白组。枳椇子葛花组小鼠较模型组小鼠肝脏组织细胞形态结构较规则,细胞核形状规则且排列整齐,炎症细胞浸润明显减少。枳椇子葛花组小鼠胃部形态较模型组完整,细胞形态良好,胃黏膜断层或破裂情况明显减少。结论 枳葛提取液对小鼠酒精性肝损伤及胃溃疡有保护作用。

Objective To investigate protective effect of extracts from pueraria lobata and hovenia dulcis on acute alcoholic liver injury and gastric ulcer of mice.Methods Except for normal group,mice in other groups were treated with corresponding intervention drugs for 4 hours every day and gavaged with 50%ethanol 10g/kg for 14 days.After the last ethanol gavage for 6 hours,mice were anesthetized to detect activities of alanine transaminase(ALT)and aspartate transaminase(AST)in serum respectively.Liver and stomach tissues were taken for histopathological observation.Results Serum levels of ALT and AST in each treatment group were significantly lower than model group(P<0.01),slightly higher than blank group.Liver tissue cell morphology and structure in hovenia dulcis and pueraria lobata group was more regular than model group,with nucleus arranged in a regular and orderly manner,infiltration of inflammatory cells was significantly lower.Morphology of stomach was complete,cell morphology was better and gastric mucosa fracture or rupture was significantly less than model group.Conclusion Extract of hovenia dulcis and pueraria lobata can achieve protective effect on alcoholic liver injury and gastric ulcer of mice.