用于蛋白输送的有机硅载体综合实验设计

Comprehensive experimental design of integrated biomaterials for organosilica-based protein delivery carrier

有机硅是杂化生物材料的典型代表和发展方向之一,基于有机硅的综合实验既涵盖了无机、有机以及高分子等材料学科内部的交叉,也包含了材料与生命科学的交叉。实验合成了活性氧响应性的有机硅烷,通过反相纳米乳液和溶胶-凝胶方法制备有机硅纳米粒子,可包载和输送绿色荧光蛋白。通过核磁共振、动态光散射、荧光成像以及流式细胞术等方法评价了其理化特性和蛋白传输等性能。载蛋白有机硅纳米粒子具有粒径小、细胞吸收效率高、活性氧响应性释放、可降解和低毒性的优点。通过多学科知识的综合应用,强化对学生交叉研究能力的训练。

The organosilica materials are a typical representative and development direction of hybrid biomaterials.The content of this integrated experiment includes not only the internal cross-studies between inorganic,organic,and polymeric materials but also the interdisciplinary studies between materials and life sciences.The reactive oxygen species(ROS)-sensitive organosilane is synthesized and used to prepare organosilica nanoparticles through sol-gel reaction in reverse nano-emulsion,which can be used to encapsulate and deliver green fluorescent protein.Their physicochemical properties and protein delivery ability are evaluated through nuclear magnetic resonance,dynamic light scattering,fluorescence imaging,and flow cytometer analyses.The protein-loaded organosilica nanoparticles has several advantages,including small diameter,high cellular uptake efficiency,ROS-sensitive protein release,biodegradability,and low cytotoxicity.These features of the integrated experiment are conducive to understanding and mastering the knowledge and technologies of hybrid biomaterials preparation and evaluation for students.