Box-Behnken法优化以豆渣粉为氮源发酵透明质酸的工艺研究

Optimization of fermentation process of hyaluronic acid using soybean residue powder as nitrogen source by Box-Behnken method

对植物氮源豆渣粉发酵透明质酸(HA)的工艺进行优化,以HA质量浓度为响应值,在单因素试验基础上,采用Box-Behnken法优化确定了最佳发酵工艺:以蒸馏水1 000 mL为基准,豆渣粉添加量30 g/L、发酵温度37℃、发酵时间24 h,在此条件下HA质量浓度最高,为0.534 g/L。

The fermentation process of hyaluronic acid(HA)from plant nitrogen source soybean residue powder was optimized.Based on single factor experiments,the Box-Behnken method was used to deter-mine the optimal fermentation process using HA mass concentration as the response value.The optimal fermentation process was determined using 1000 mL of distilled water as the benchmark,with 30 g/L ad-dition of soybean residue powder,fermentation temperature of 37 ℃,and fermentation time of 24 h.Un-der these conditions,the highest HA mass concentration was 0.534 g/L.