血清中DNA聚合酶α在阿尔茨海默病中的表达和诊断价值分析

Expression and diagnostic efficacy of serum DNA polymeraseαin Alzheimer′s disease

目的探究血清中DNA聚合酶α(DNA polα)在阿尔茨海默病(AD)中的表达水平,分析其在AD中的诊断价值。方法选取2019年3月至2023年4月在首都医科大学宣武医院就诊的AD痴呆(DAT)期患者100例作为DAT组,轻度认知障碍(MCI)期患者43例作为MCI组;同期,选取68例同年龄组别的健康者作为HC组。检测各组血清样本中DNA polα的表达水平,利用受试者工作特征(ROC)曲线分析DNA polα在AD中的诊断价值。结果DAT组血清DNA polα表达水平高于MCI组(P<0.05)和HC组(P<0.001),随着AD病程发展,DNA polα表达水平有递增趋势。DNA polα表达水平与简易智能精神状态检查量表(MMSE)和蒙特利尔认知评估量表(MoCA)评分均呈负相关(r=-0.1553、-0.2037,P<0.05)。DNA polα诊断DAT期的曲线下面积(AUC)为0.682,灵敏度为0.900,特异度为0.412;DNA polα诊断MCI期的AUC为0.546,灵敏度为0.977,特异度为0.250;DNA polα鉴别诊断MCI期和DAT期的AUC为0.664,灵敏度为0.780,特异度为0.535。结论DNA polα在DAT期AD患者中表达水平升高,且其表达水平与AD病程发展存在一定联系,推测DNA polα有可能是诊断AD潜在的血液生物标志物。

Objective To investigate the expression level and diagnostic value of serum DNA polymeraseα(DNA polα)in Alzheimer′s disease(AD),and analyze its diagnostic efficacy in AD.Methods A total of 100 patients of dementia of Alzheimer′s type(DAT)and 43 patients of mild cognitive impairment(MCI)from Xuanwu Hospital Capital Medical University from March 2019 to April 2023 were included in this study,and 68 healthy individuals of the same age group were collected as the HC group.The expression level of DNA polαwas detected in each group,and the diagnostic value of DNA polαin AD was analyzed by receiver operating characteristic(ROC)curve.Results The expression level of DNA polαin DAT group was higher than those in the MCI group(P<0.05)and the HC group(P<0.001).The expression level of DNA polαshowed an increasing trend as AD progressed.The expression level of DNA polαwas negatively correlated with Mini Intelligent Mental State Examination Scale(MMSE)score and Montreal Cognitive Assessment Scale(MoCA)score(r=-0.1553,-0.2037,P<0.05).The area under the curve(AUC)of DNA polαfor diagnosing DAT was 0.682,and the sensitivity was 0.900.The AUC for diagnosing MCI was 0.546,and the sensitivity was 0.977.The AUC for differential diagnosis of MCI and DAT was 0.664,the sensitivity was 0.780,and the specificity was 0.535.Conclusion The expression level of DNA polαis significantly increased in AD patients with DAT,and its expression level is related to the progression of AD,suggesting that DNA polαhas the possibility to be a potential blood biomarker for the diagnosis of AD.