摘要
大肠杆菌O157:H7(Escherichia coli O157:H7)是一种重要的与公共卫生相关的食源性病原体。抗体分子是体液免疫应答中最重要的效应分子,具有多种生物学活性,最主要的生物学功能是与相应抗原特异性结合。适配体是体外合成的较短DNA序列,可通过识别特定区域和靶标特异性结合。利用抗体和适配体的特异性识别功能及免疫磁珠的捕获作用和磁分离,并通过多克隆抗体和裁剪得到的适配体搭建生物传感器,使用实时荧光定量聚合酶链式反应(real-time fluorescence quantitative polymerase chain reaction,qPCR)可以实现对靶标在(8×10^(3))-(8×10^(6))CFU/mL范围内的定量检测,检测限为800 CFU/mL。
Escherichia coli O157:H7 is an important food borne pathogen related to public health.Antibody molecules are the most important effector molecules in the humoral immune response.They have a variety of biological activities.The main biological function is to specifically bind to the corresponding antigen.Aptamers are shorter DNA sequences synthesized in vitro,which can bind specifically by recognizing specific regions and targets.Based on the specific recognition function of antibody and aptamer,the capture effect and magnetic separation of immunomagnetic beads,and the construction of biosensor by polyclonal antibody and tailoring aptamer,real-time fluorescence quantitative polymerase chain reaction(qPCR)may achieve the detection of target at(8×10^(3))-(8×10^(6))CFU/mL.The limit of detection is 800 CFU/mL for quantitative detection.
作者
侯炜辰
叶柯
李洁
张洋子
许文涛
朱龙佼
李相阳
HOU Wei-chen;YE Ke;LI Jie;ZHANG Yang-zi;XU Wen-tao;ZHU Long-jiao;LI Xiang-yang(Beijing Laboratory of Food Quality and Safety,Beijing Key Laboratory of Detection and Control of Spoilage Organisms and Pesticide Residue in Agricultural Product,College of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083;Key Laboratory of Precision Nutrition and Food Quality,Department of Nutrition and Health,China Agricultural University,Beijing 100083)
出处
《生物技术通报》
CAS
CSCD
北大核心
2023年第12期81-89,共9页
Biotechnology Bulletin
基金
国家重点研发计划(2022YFF0607900)
北京市景观休闲农业创新团队项目(BAIC09-2023)
北京市科技计划(Z221100007122004)。
