广西产火炭母总黄酮提取工艺优化及含量测定

Optimization of extraction process and content determination of total flavonoids from Polygonum chinense L.produced in Guangxi

采用紫外可见分光光度法测定火炭母(Polygonum chinense L.)中总黄酮的含量,以总黄酮含量为考察指标,以提取方式、提取溶剂、料液比、提取时间、提取次数为考察因素,在单因素试验的基础上,采用L9(33)正交设计优化火炭母中总黄酮的提取工艺。最优提取工艺:70%乙醇为提取溶剂,料液比1∶70(m/V),超声波提取45 min,提取2次。以芦丁为对照品,在500 nm波长处测定其吸光度,平均加样回收率为98.76%,相对标准偏差为1.72%。结果表明,13个产地火炭母药材的总黄酮含量为22.205 4~52.173 7 mg/g,相对标准偏差(RSD)为1.07%~2.81%。13个产地总黄酮含量由高到低依次为桂林市、南宁市、玉林市、百色市、贺州市、河池市、贵港市、防城港市、钦州市、梧州市、来宾市、崇左市、柳州市。该方法精密度、稳定性、重复性良好,可作为广西产火炭母药材质量评价的依据。

The content of total flavonoids in Polygonum chinense L.was determined by UV visible spectrophotometry.The content of to-tal flavonoids was used as the evaluation index,and the extraction method,solvent,solid-liquid ratio,extraction time,and extraction frequency were used as the evaluation factors,on the basis of single factor experiments,L9(33)orthogonal design was used to optimize the extraction process of total flavonoids in Polygonum chinense L..The optimal extraction process was 70%ethanol extraction solvent,with a solid-liquid ratio of 1∶70(m/V),ultrasonic extraction for 45 minutes,and extraction twice.Using rutin as a reference sub-stance,its absorbance was measured at a wavelength of 500 nm,with an average recovery rate of 98.76%and a relative standard devia-tion of 1.72%.The results showed that the total flavonoids content of Polygonum chinense L.from 13 production areas ranged from 22.2054 to 52.1737 mg/g,with a relative standard deviation(RSD)of 1.07%to 2.81%.The total flavonoids content in 13 production areas was ranked as follows:Guilin City,Nanning City,and Yulin City,Baise City,Hezhou City,Hechi City,Guigang City,Fangcheng-gang City,Qinzhou City,Wuzhou City,Laibin City,Chongzuo City,Liuzhou City from high to low.This method had good precision,sta-bility,and repeatability,and could be used as a basis for the quality evaluation of Polygonum chinense L.produced in Guangxi.