外周血miR-4465和miR-6089在亚临床糖尿病性心肌病诊断中的价值

Analysis of value of peripheral blood miR-4465 and miR-609 detection in the diagnosis of subclinical disease progression

目的评估外周血miR-4465和miR-6089水平诊断亚临床糖尿病性心肌病(DCM)的临床价值。方法选择266例亚临床Ⅱ型糖尿病受试者,通过超声心动图检查,49例患者被选入DCM组,另选取49例非DCM患者纳入对照组。单因素和多因素Logistic回归分析DCM发生的危险因素。ROC评价miR-4465和miR-6089对DCM的诊断价值。Pearson相关性分析miR-4465、miR-6089与糖尿病病程、糖化血红蛋白(HbA1C)的相关性。结果DCM组糖尿病病程长于对照组,HbA1c水平高于对照组,外周血miR-4465和miR-6089水平低于对照组(均P<0.05)。多因素Logistic回归分析结果显示,糖尿病病程、HbA1c、外周血miR-4465和miR-6089均为DCM的危险因素(P<0.05)。外周血miR-4465和miR-6089对DCM具有良好的诊断价值。Pearson相关性分析显示糖尿病病程、HbA1c均与外周血miR-4465和miR-6089呈负相关(P<0.05)。结论外周血miR-4465和miR-6089为DCM的危险因素,且与糖尿病病程和HbA1c呈负相关,可以作为诊断DCM的生物学标志物。

Aim To evaluate the clinical value of peripheral blood miR-4465 and miR-6089 levels in the diagnosis of subclinical diabetes cardiomyopathy(DCM).Methods 266 subjects with type II diabetes were selected.According to echocardiography,49 patients were selected as the DCM group,and 49 non-DCM patients were selected as the control group.Single factor and multiple factor logistic regression analysis were used to identify the risk factors affecting the occurrence of DCM.ROC was used to calculate the diagnostic value of miR-4465 and miR-6089 for DCM.Pearson correlation analysis was used to indicate the correlation between indicators.Results The duration of diabetes in DCM group was longer than that in control group,the level of HbA1c was higher than that in control group,and the levels of miR-4465 and miR-6089 in peripheral blood were lower than that in control group(P<0.05).Multivariate logistic regression analysis showed that the course of diabetes,HbA1c,peripheral blood miR-4465 and miR-6089 were all risk factors for the occurrence of DCM in diabetes(all P<0.05).Peripheral blood miR-4465 and miR-6089 have good diagnostic value for DCM.Pearson correlation showed that the course of diabetes and HbA1c were significantly correlated with miR-4465 and miR-6089 in peripheral blood(P<0.05).Conclusion Peripheral blood miR-4465 and miR-6089 are risk factors of diabetes DCM,and have significant correlation with the course of diabetes and HbA1c,which can be used as new biomarkers for the diagnosis of DCM.