烟草抗感南方根结线虫品种根部差异表达mRNA的PPI网络分析

PPI network analysis of differentially expressed mRNA in the roots of tobacco varieties resistant to Meloidogyne incongnita (Kofold&White) Chitwood

基于品种间的差异表达基因,选取烟草抗病品种K326与感病品种长脖黄为试验材料,利用生物信息学分析方法筛选出与抗感染相关的关键基因,通过蛋白互作网络分析(Protein-Protein Interaction,PPI)以及Gene Ontology(GO)功能分类分析(包括Biological Process分析与KEGG通路分析)对差异表达基因进行生物学功能注释,并找出PPI网络中的重要基因以及作用通路一致的基因,并对这些基因加以解析,以明确抗南方根结线虫烟草品种抗性机制,为研究烟草抗南方根结线虫的分子机制提供理论依据。结果表明,差异表达基因主要在细胞代谢、胞内体转运、RNA介导的基因沉默等9条与抗感染相关的通路中显著富集,并且筛选出Fab1b、Fab1c、Fab1d、Glu1、Dcl1、Dcl2、Dcl4共7个同时构成PPI网络的关键基因。

Based on the differentially expressed genes among varieties,tobacco disease resistant variety K326 and susceptible variety Changbohuang were selected as experimental materials,and key genes related to infection resistance were screened using bioinformatics analysis methods,this article used Protein Protein Interaction(PPI) and Gene Ontology(GO) functional classification analysis(including Biological Process analysis and KEGG pathway analysis) to annotate the biological functions of differentially expressed genes,identify important genes and genes with consistent pathways in the PPI network,analyze these genes to clarify the resistance mechanism of tobacco varieties to Meloidogyne incongnita(Kofold&White) Chitwood,and provide theoretical basis for studying the molecular mechanism of tobacco resistance to Meloidogyne incongnita(Kofold&White) Chitwood.The results showed that differentially expressed genes were significantly enriched in 9 pathways related to anti-infection,including cell metabolism,intracellular transport,and RNA mediated gene silencing.Seven key factor genes that simultaneously formed the PPI network were identified,including Fab1b,Fab1c,Fab1d,Glu1,Dcl1,Dcl2 and Dcl4.