IFN-γ表达与食管癌患者细胞免疫水平及预后的相关性分析

Relationships of IFN-γ expression with the immune levels and prognosis of patients with esophageal cancer

目的:探讨干扰素-γ(interferon gamma,IFN-γ)与食管癌患者细胞免疫水平及预后的相关性。方法:收集40例食管癌患者外周血标本,流式细胞仪检测标本中IFN-γ的浓度及CD4+T/CD8+T细胞比值;利用TISIDB数据库分析IFN-γ表达与免疫调控基因的关系;收集119例食管癌患者临床预后信息,利用Kaplan-Meier生存曲线分析IFN-γ表达与食管癌患者生存预后的相关性。结果:外周血中IFN-γ的浓度和CD4+T/CD8+T细胞比值呈负相关(R2=0.1662,P=0.009);食管癌组织中IFN-γ表达与免疫负调控基因CD274(rho=0.449,P<0.001)和PDCD1(rho=0.778,P<0.001)的表达呈正相关;IFN-γ高表达于食管癌组织(P<0.001),且IFN-γ高表达组患者的总生存(overall survival,OS)显著低于IFN-γ低表达组(P=0.008)。结论:IFN-γ在食管癌组织中的表达与细胞免疫水平呈负相关,同时可作为食管癌患者预后不良的指标。

Objective:To analyze the relationship of IFN-γ expression with the immune levels and prognosis of patients with esophageal cancer.Methods:A total of 40 patients with esophageal cancer were enrolled,and serum IFN-γ levels and the absolute values of CD4+/CD8+T cells were detected by flow cytometry.Then,the TISIDB database was used in analyzing the effects of IFN-γ on immunoregulatory genes.The relationship between IFN-γ expression level and prognosis in 119 patients with esophageal cancer was statistically analyzed using a Kaplan-Meier curve.Results:A negative correlation was found between IFN-γ expression in serum and CD4^(+)/CD8^(+)T cells ratio(R 2=0.1662,P=0.009),and IFN-γ expression was positively correlated with the expression of CD274(rho=0.449,P<0.001)and PDCD1(rho=0.778,P<0.001).The expression of IFN-γ was elevated in esophageal cancer tissues compared with the normal tissues(P<0.001).The overall survival(OS)of IFN-γ over-expression patients was shorter than those in IFN-γ low-expression group(P=0.008).Conclusion:The expression of IFN-γ was negatively correlated to immune level and might be a novel predictor for the poor prognosis of esophageal cancer patients.