龟羚帕安丸对帕金森病大鼠血清NSE、Cys-C、5-HT、5-HIAA及NE水平的影响

Effects of Guiling Paan Pill(龟羚帕安丸)on Serum Neuron-specific Enolase,Cystatin C,5-hydroxytryptamine,5-hydroxyindoleaceticAcid and Norepinephrine in Enzyme Linked Immunosorbent Assay Rats

目的观察龟羚帕安丸对帕金森病(Parkinson’s disease,PD)大鼠血清神经元特异性烯醇化酶(Neuron-specific enolase,NSE)、胱抑素C(Cystatin C,Cys-C)、5-羟色胺(5-Hydroxytryptamine,5-HT)、5-羟基吲哚乙酸(5-Hydroxyindoleacetic acid,5-HIAA)及去甲肾上腺素(Norepinephrine,NE)水平的影响。方法采用6-羟基多巴胺(6-OHDA)立体定向注射法制作PD大鼠模型,造模成功的PD大鼠随机分为模型组,西药组,中药高、中、低剂量组,中西药合用组,每组15只,另设假手术组15只。模型组及假手术组给予等容积生理盐水灌胃,其余组给予相应药物灌胃,连续给药28 d。大鼠腹主动脉取血,用酶联免疫吸附测定法(Enzyme linked immunosorbent assay,ELISA)检测各组大鼠血清NSE、Cys-C、5-HT、5-HIAA及NE的水平。结果模型组大鼠血清NSE及Cys-C水平均明显升高,血清5-HT、5-HIAA及NE水平均明显降低;中药各剂量组、中西药合用组、西药组血清NSE及Cys-C水平均明显降低,5-HT、5-HIAA及NE水平均明显升高。结论龟羚帕安丸具有明显神经保护作用,作用机制与降低NSE及Cys-C水平,增加5-HT、5-HIAA及NE水平,降低脑实质损伤、神经炎症损伤及提高单胺类神经递质有关。

Objective To observe the effects of Guiling Paan Pill(龟羚帕安丸)on serum levels of neuron-specific enolase(NSE),Cystatin C(Cys-C),5-hydroxytryptamine(5-HT),5-hydroxyindoleacetic acid(5-HIAA)and norepinephrine(NE)in Parkinson's disease(PD)rats.Methods The PD rat model was made by injection of 6-hydroxydopamine(6-OHDA).The successful PD rats were randomly divided into model group(n=15),western medicine group(n=15),high dose group(n=15),middle dose group(n=15),low dose group(n=15)and combination group(n=15).The model group and sham operation group were given the same volume of normal saline,and the other groups were given corresponding drugs for 4 weeks.The serum levels of NSE,Cys-C,5-HT,5-HIAA and NE were detected by enzyme linked immunosorbent assay(ELISA).Results In the model group,the levels of serum NSE and Cys-C were significantly increased,while the levels of 5-HT,5-HIAA and NE were significantly decreased.The levels of serum NSE and Cys-C in each dose group of traditional Chinese medicine,the combination group of traditional Chinese and Western medicine and the Western medicine group were significantly reduced while the levels of 5-HT,5-HIAA and NE were significantly increased.Conclusion Guiling Paan Pill has obvious neuroprotective effect,which is related to the decrease of the levels of NSE and Cys-C,the increase of 5-HT,5-HIAA and NE,the decrease of brain parenchyma injury,nerve inflammatory injury and the increase of monoamine neurotransmitters.