锥栗半同胞家系栗疫病抗性与生长性状的遗传分析

Genetic analysis of chestnut blight resistance and growth traits for half-sib families of Castanea henryi

为筛选出生长良好且栗疫病抗性强的优良家系和优良单株,对12个锥栗半同胞家系的栗疫病抗性相关性状及生长性状进行变异分析、方差分析、相关性分析和遗传参数估算。结果表明,12个锥栗半同胞家系各性状的平均变异系数为20.19%,病斑面积的变异系数最大,为39.76%,叶形指数的变异系数最小,为11.14%。病斑宽、叶面积、叶周长、叶长、叶脉数在家系间具有显著差异(P<0.05),感病率、感病指数、叶宽在家系间具有极显著差异(P<0.01)。苗高与感病率呈显著负相关(P<0.05),与感病指数呈极显著负相关(P<0.01);叶长与感病率、感病指数呈极显著负相关(P<0.01)。筛选出JO14('白露仔’)、JO8('油桐仔’)、JO_(2)('大苞榛’)3个优良家系;筛选出9株(JO_(2)-25、JO13-29、JO14-32、JO_(2)-12、JO8-17、JO_(3)-4、JO5-26、JO_(2)-19、JO12-20)栗疫病抗性强的优良单株,4株(JO_(2)-5、JO7-16、JO8-17、JO14-3)生长良好的优良单株,其中JO8-17抗性强且生长良好。

Variation,variance,and correlation analyses and estimations of genetic parameters of traits related to chestnut blight resistance and growth in half-sibs of Castanea henryi were performed to screen for excellent families,individuals with good growth,and strong resistance to chestnut blight.The results showed that the average coefficient of variation(CV)of traits in 12 half-sib families was 20.19%,with the CV of the lesion area being the largest(39.76%)and that of the leaf shape index being the smallest(11.14%).Among the families,there were significant differences in lesion width,leaf area,leaf perimeter,leaf length,and vein number(P<0.05)and highly significant differences in infection rate,infection index,and leaf width(P<0.01).The seedling height showed a significant negative correlation with the infection rate(P<0.05)and a highly significant negative correlation with the infection index(P<0.01).The leaf length exhibited a highly significant negative correlation with the infection rate and index(P<0.01).Three excellent families were selected;namely,JO14('Bailuzi'),JO8('Youtongzi'),and JO_(2)('Dabaozhen').Nine individuals(JO_(2)-25,JO13-29,JO14-32,JO_(2)-12,JO8-17,JO_(3)-4,JO5-26,JO_(2)-19,and JO12-20)with strong resistance and four individuals(JO_(2)-5,JO7-16,JO8-17,and JO14-3)with good growth were selected,among which JO8-17 showed strong resistance and excellent growth.