摘要
目的探究三七总皂苷对脑缺血再灌注后神经功能恢复的影响。方法培养30只SPF级雄性SD大鼠,构建脑缺血再灌注模型。将大鼠分为假手术组、模型组和三七总皂苷组,每组10只大鼠。检测各组大鼠脑梗面积、脑含水量。Longa评分评价大鼠神经功能缺损情况。水迷宫实验检测大鼠神经功能。检测各组大鼠血清中氧化应激指标超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)、谷光甘肽(glutathione,GSH)含量和炎症因子指标肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)、白细胞介素-10(interleukin-10,IL-10)含量。Tunel染色检测各组大鼠海马神经元细胞凋亡情况。结果和假手术组大鼠相比,模型组大鼠脑梗面积、脑含水量、神经功能缺损评分、学习潜伏期和记忆潜伏期显著升高,SOD、GSH、IL-10含量显著降低,MDA、TNF-α、IL-1β含量显著升高,海马神经元凋亡比例显著升高(P<0.05)。和模型组大鼠相比,三七总皂苷组大鼠脑梗面积、脑含水量、神经功能缺损评分、学习潜伏期和记忆潜伏期显著降低,SOD、GSH、IL-10含量显著升高,MDA、TNF-α、IL-1β含量显著降低,海马神经元凋亡比例显著降低(P<0.05)。结论三七总皂苷可能通过抑制大鼠炎症反应和氧化应激,减少海马神经元凋亡,促进脑缺血再灌注后神经功能恢复。
Objective To investigate the effect of Sanqi(Panax notoginseng)total saponin on the recovery of neurological function after cerebral ischemia-reperfusion.Methods Thirty SPF male SD rats were cultured and the cerebral ischemia-reperfusion model was established.The rats were divided into sham operation group,model group and Sanqi(Panax notoginseng)total saponin group,with 10 rats in each group.The cerebral infarction area and brain water content were measured.The neurological deficit of rats was evaluated by Longa score.The nerve function of rats was detected by water maze test.The contents of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH)and the levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β)and interleukin-10(IL-10)were measured.TUNEL staining was used to detect the apoptosis of hippocampal neurons in each group.Results Compared with those of the sham operation group,the cerebral infarction area,brain water content,neurological deficit score,learning latency and memory latency of the model group increased significantly,the contents of SOD,GSH and IL-10 decreased significantly,the contents of MDA,TNF-αand IL-1βincreased significantly,and the proportion of hippocampal neuron apoptosis increased significantly(P<0.05).Compared with those of the model group,the cerebral infarction area,brain water content,neurological deficit score,learning latency and memory latency of Sanqi(Panax notoginseng)total saponin group decreased significantly,the contents of SOD,GSH and IL-10 increased significantly,the contents of MDA,TNF-αand IL-1βdecreased significantly,and the apoptosis of hippocampal neurons decreased significantly(P<0.05).Conclusion Sanqi(Panax notoginseng)total saponin may reduce hippocampal neuron apoptosis and promote the recovery of neural function after cerebral ischemia-reperfusion by inhibiting inflammatory response and oxidative stress in rats.
作者
肖新宇
张瑾
李秀华
高艾东
孙齐美
王天一
郝旭丽
XIAO Xinyu;ZHANG Jin;LI Xiuhua;GAO Aidong;SUN Qimei;WANG Tianyi;HAO Xuli(Tangshan Workers′Hospital,Tangshan 063000,Hebei,China;Tangshan Central Hospital,Tangshan 063000,Hebei,China;Affiliated Hospital of North China University of Science and Technology,Tangshan 063000,Hebei,China)
出处
《辽宁中医杂志》
CAS
北大核心
2024年第3期187-190,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
河北省中医药管理局科研计划项目(2021412)。
关键词
三七总皂苷
脑缺血再灌注
神经功能
炎症反应
氧化应激
Sanqi(Panax notoginseng)total saponin
cerebral ischemia reperfusion
nerve function
inflammatory response
oxidative stress