Construction and validation of prognostic model of Cuproptosis-related LncRNA in osteosarcoma

Cuproptosis is a newly discovered form of apoptotic process that is thought to play an important role in cancer therapy.Long non-coding RNA(lncRNA)is involved in regulating many physiological and pathological activities of cells.The aim of this study was to investigate the prognostic significance of Cuproptosis-associated lncRNAs in osteosarcoma.Methods:The Gene expression profiling of osteosarcoma samples versus normal samples and corresponding clinical data were downloaded from the public databases UCSC Xena and GTEx,and the cuproptosis gene was obtained from the published literature,the prognostic model of osteosarcoma cuproptosis-related lncRNA was constructed by using coexpression network,minimum absolute contraction and selection algorithm(LASSO)and Cox regression model.Receiver operating characteristic(ROC)curves and nomograms were used to assess the predictive power of the model.Single-sample gene set enrichment analysis(ssGSEA)was used to explore the relationship between osteosarcoma immune cells and function in different risk groups.Results:181 cuproptosis-related lncRNAs were obtained by co-expression analysis of 19 cuproptosis genes collected.Ten lncRNAs were screened out by differential analysis and single-factor Cox analysis.Three cuproptosis-related lncrnas(AC124798.1,AC090152.1,AC090559.1)were screened by Lasso and multivariate Cox regression to construct the prognostic model.Patients were divided into high and low risk groups based on the median risk score.The results of overall survival,risk score distribution and survival status in the lowrisk group were better than those in the high-risk group,and were verified in the internal data.Univariate and multivariate Cox regression analyses showed that risk score was an independent prognostic factor.Nomograms and ROC curves showed that the prognostic model had good predictive ability.The results of ssGSEA suggest that immune cells and function may be inhibited in the high-risk group.Conclusion:The 3 cuproptosis-related lncRNAs may be helpful to guide the prognosis of osteosarcoma patients and provide some theoretical basis for clinical decision.