神经节苷脂对脑缺氧缺血后新生大鼠海马CA_1区N-甲基-D-天门冬氨酸受体表达的影响

Effect of gangliosides on N-methy-D-aspartate receptor expression in hippocampal CA_1 in neonatal rat models with cerebral hy-poxic-ischemia

目的 研究新生大鼠缺氧缺血性脑损伤(HIBD)后海马区N-甲基-D-天门冬氨酸受体(NMDAR)及NMDARmRNA表达的变化和神经节苷脂(GM1)对其的影响。方法 建立HIBD模型。用免疫组化及原位杂交方法检测缺氧缺血(HI)和GM1干预后不同时间点NMDA受体I型亚单位(NR1)阳性细胞及NR1mRNA阳性细胞的表达。结果 HI后新生大鼠海马CA1区NR1及NR1mRNA表达增强,GM1可使其受体表达下调(P<0.05)。结论 GM1可使新生大鼠海马CA1区NR1及NR1mRNA表达下调,对新生大鼠缺氧缺血性脑损伤具有保护作用。

Objective To study the expression of N-methy-D-aspartate receptor(NMDAR) and NMDAR mRNA in hip-pocampal CA1 and the effect of monosialotetrahexosylganglioside(GM1) on the expression after hypoxic-ischemic brain damage(HIBD) in neonatal rats. Methods An animal model of neonatal hypoxic-ischemic brain damage was established. The expression of NR1 positive cells was detected with NR1 immunohistochemisry and NR1 mRNA in situs hybridization. Results The expression of positive cells was observed in hippocampal CA1 in neonatal rats NR1 positive cells and NR1 mRNA positive cells were increased in hippocampal CA1 area after hypoxic-ischemia (HI). The expression of NR1 and NR1 mRNA was down-regulated after GM1 administration in GM1 group compared to that of HI group. Conclusion GM1, as antagonist of the NMDAR, plays a role in brain protection against hypoxic-ischemia.