DCLK1和ITGA5在非小细胞肺癌患者中的表达及其与临床病理特征及预后的关系研究

Expression of DCLK1 and ITGA5 in non-small cell lung cancer patients and their relationship with clinical pathological characteristics and prognosis

目的 检测非小细胞肺癌(non-small cell lung cancer,NSCLC)患者血清双皮质素样激酶1(doublecortin-like kinase 1,DCLK1)和整合素α5(integrin alpha5,ITGA5)水平并讨论二者与临床病理特征及预后的关系。方法 选取2017年6月1日至2020年7月1日在我院收治的116例NSCLC患者(NSCLC组)作为研究对象,收集同期100例健康受试者作为对照组,采用酶联免疫吸附法检测NSCLC组和对照组受试者血清中DCLK1和ITGA5的水平;根据预后情况将患者分为生存组和死亡组,ROC曲线分析血清DCLK1、ITGA5的水平对NSCLC患者3年发生死亡的预测价值,多因素Cox回归分析影响NSCLC患者发生死亡的因素。结果 NSCLC组患者血清DCLK1、ITGA5的水平高于对照组(P<0.05);不同吸烟史、TNM分期、分化程度、肿瘤直径以及淋巴结转移的NSCLC患者血清DCLK1、ITGA5的水平比较,差异有统计学意义(P<0.05);死亡组患者血清DCLK1、ITGA5的水平均高于生存组(P<0.05);血清DCLK1、ITGA5单独及联合预测患者发生死亡的AUC分别为0.895(95%CI=0.824~0.944)、0.828(95%CI=0.747~0.892)、0.926(95%CI=0.862~0.966);DCLK1、ITGA5及淋巴结转移是影响NSCLC患者发生死亡的独立危险因素(P<0.05)。结论 NSCLC患者血清DCLK1、ITGA5的水平异常升高,二者的表达水平与NSCLC患者临床病理特征和不良预后有关。

Objective To detect serum doublecortin-like kinase 1(DCLK1) and integrin alpha5(ITGA5) levels in patients with non-small-cell lung cancer(NSCLC),and to explore their relationship with clinicopathological features and prognosis.Methods A total of 116 NSCLC patients admitted to our hospital from June 1,2017 to July 1,2020 were selected as the NSCLC group,and 100 healthy subjects in the same period were collected as the control group.Their serum levels of DCLK1 and ITGA5 were detected by ELISA.The patients were divided into the survival group and the death group according to prognosis.Receiver operator characteristic(ROC) curve was used to analyze the predictive value of serum DCLK1 and ITGA5 levels on 3-year death in NSCLC patients.Multivariate Cox regression was used to analyze the factors affecting death in NSCLC patients.Results The serum levels of DCLK1 and ITGA5 in the NSCLC group were higher than those in the control group(P<0.05).There were statistically obvious differences in serum levels of DCLK1 and ITGA5 among NSCLC patients with different smoking history,TNM staging,degree of differentiation,tumor diameter,and lymph node metastasis(P<0.05).The levels of serum DCLK1 and ITGA5 in the death group were higher than those in the survival group(P<0.05).Serum DCLK1 and ITGA5 alone and in combination predicted death The AUC values were 0.895(95%CI=0.824~0.944),0.828(95%CI=0.747-0.892) and 0.926(95%CI=0.862~0.966),respectively.DCLK1,ITGA5,and lymph node metastasis were independent risk factors for mortality in NSCLC patients(P<0.05).Conclusion Serum levels of DCLK1 and ITGA5 in NSCLC patients were abnormally elevated,and their expression levels were associated with clinicopathological features and poor prognosis of NSCLC patients.