多发性子宫肌瘤患者血清ANGPTL2、VASH1的表达及临床意义

The expression and clinical significance of serum ANGPTL2 and VASH1 in patients with multiple uterine fibroids

目的 分析血清中血管生成素样蛋白2(ANGPTL2)和血管生成抑制蛋白1(VASH1)的表达水平,探讨其在多发性子宫肌瘤中的临床意义。方法 选取2018年1月至2022年1月于东南大学医学院附属南京同仁医院就诊的312例多发性子宫肌瘤患者为研究对象(观察组),同时期来本院体检的312名健康女性作为对照(对照组);酶联免疫吸附试验(ELISA)检测血清中ANGPTL2和VASH1水平;Pearson相关分析血清中ANGPTL2和VASH1水平的相关性;受试者工作特征(ROC)曲线分析血清中ANGPTL2和VASH1水平对多发性子宫肌瘤的诊断价值;Logistic回归分析多发性子宫肌瘤发生的影响因素。结果 观察组血清中ANGPTL2和VASH1水平显著高于对照组,差异有统计学意义(t=12.870、9.935,P<0.05);血清中ANGPTL2和VASH1水平与阴道不规则出血、肌瘤数量、最大肌瘤直径以及平均肌瘤体积有关,差异有统计学意义(P<0.05),而与年龄、月经情况、绝经情况、妊娠史、流产史、ER、PR以及肿瘤部位无关,差异无统计学意义(P>0.05);Pearson相关分析结果显示,多发性子宫肌瘤患者血清中ANGPTL2和VASH1水平呈正相关(r=5.440,P<0.05);ROC曲线分析结果显示,血清中ANGPTL2和VASH1水平联合诊断多发性子宫肌瘤的曲线下面积(AUC),效果较ANGPTL2和VASH1单一指标更好(P<0.05);Logistic回归分析结果显示,ANGPTL2、VASH1是多发性子宫肌瘤发生的影响因素(P<0.05)。结论 多发性子宫肌瘤患者血清中ANGPTL2和VASH1水平显著升高,两者联合可辅助诊断多发性子宫肌瘤。

Objective To analyze the expression levels of angiopoietin-like protein 2(ANG-PTL2)and vasohibin-1(VASH1)in serum,and to explore their clinical significance in patients with multiple uterine fibroids.Methods A total of 312 patients with multiple uterine fibroids who were treated at the Nan-jing Tongren Hospital affiliated with the School of Medicine of Southeast University from January 2018 to Jan-uary 2022 were included as study subjects(observation group),312 healthy women who visited our hospital for physical examinations were enrolled as controls(control group).Enzyme linked immunosorbent assay(ELISA)was used to measure the levels of ANGPTL2 and VASH1 in serum.Pearson correlation analysis was conducted to examine the relationship between the levels of ANGPTL2 and VASH1 in serum.Receiver operat-ing characteristic(ROC)curve analysis was performed to assess the diagnostic value of serum ANGPTL2 and VASH1 levels for multiple uterine fibroids.Logistic regression was employed to identify the influencing factors of multiple uterine fibroids.Results The levels of ANGPTL2 and VASH1 in the serum of the observation group were significantly higher than those in the control group(t=12.870,9.935,P<0.05).Additionally,the levels of ANGPTL2 and VASH1 in the serum were found to be associated with irregular vaginal bleeding,number of fibroids,maximum fibroid diameter,and average fibroid volume,with statistically significant dif-ference(P<0.05).However,there was no significant association between the levels of ANGPTL2 and SASH1 and age,menstrual status,menopause status,pregnancy history,abortion history,ER,PR and tumor loca-tion(P>0.05).Pearson correlation analysis revealed a positive correlation between the levels of ANGPTL2 and VASH1 in the serum of patients with multiple uterine fibroids(r=5.440,P<0.05).The ROC curve analy-sis results showed that the area under the curve(AUC)of the combination of ANGPTL2 and VASH1 in the di-agnosis of multiple uterine fibroids,which was better than that of ANGPTL2 or VASH1 alone(P<0.05).Lo-gistic regression analysis demonstrated that ANGPTL2 and VASH1 were significant influencing factors in the occurrence of multiple uterine fibroids(P<0.05).Conclusion The levels of ANGPTL2 and VASH1 in the se-rum of patients with multiple uterine fibroids are significantly elevated.The combined measurement of there two markers can assist in the diagnosis of multiple uterine fibroids.