鄂药挨姆末的化学成分及细胞毒作用研究

Chemical constituents of Lonicera caerulea var.edulis and their cytotoxicity in HepG2 cells

目的研究鄂伦春族民族药挨姆末叶(Lonicera caerulea var.edulis)的化学成分及细胞毒作用。方法用正相柱色谱、反相柱色谱、SephadexLH-20柱色谱、HPLC等现代色谱技术对挨姆末叶的提取物进行分离纯化,通过各种波谱技术结合理化性质鉴定化合物的结构,用HepG2细胞模型评价样品的细胞毒作用。结果从鄂药挨姆末叶中分离得到12个化合物,分别鉴定为ferna-7,9(11)-diene-3α,16α-dio(l 1);3β,23-dihydroxy-olean-12-en-28-oic acid(2);2(-3-methoxy-4-hydroxyphenyl)-propane-1,3-dio(l 3);nicotinic acid(4);4-hydroxy-5-methoxy-benzoic acid(5);3,7-dihydro-1,3,7-trimethyl-1H-purine-2,6-dione(6);caffeic acid methyl este(r 7);(2E)-2,6-dimethyl-6-hydroxy-2,7-octadienoic acid(8);syringin(9);threo-5-hydroxy-3,7-dimethoxy phenylpropane-8,9-dio(l 10);3,4,5-trimethoxyphenyl 1-α-glucofuranosid(e 11);benzyl-β-D-glucosid(e 12)。其中化合物1对HepG2细胞有一定的细胞毒作用。结论这12个化合物中包括2个三萜、2个生物碱、3个木脂素、1个不饱和脂肪酸和4个芳香族化合物,化合物3、8、10、11为首次从该属中分离得到,化合物1、2、4、6、7、12为首次从该种中分离得到。

Objective To study the chemical constituents of Lonicera caerulea var.edulis and their cytotoxic activities in HepG2 cells.Methods The extract of L.caerulea was isolated and purified by normal phase column chromatography,reverse phase column chromatography,Sephadex LH-20 chromatography,and HPLC,etc.The structures of compounds were elucidated by detailed spectroscopic analysis based on physicochemical properties of the compounds.MTT method was used to evaluate their cytotoxicity in HepG2 cells.Results Twelve compounds were isolated and determined as ferna-7,9(11)-diene-3α,16α-diol(1),3β,23-dihydroxyolean-12-en-28-oic acid(2),2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-dio(l 3),nicotinic acid(4),4-hydroxy-5-methoxybenzoic acid(5),3,7-dihydro-1,3,7-trimethyl-1H-purine-2,6-dione(6),caffeic acid methyl ester(7),(2E)-2,6-dimethyl-6-hydroxy-2,7-octadienoic acid(8),syringin(9),threo-5-hydroxy-3,7-dimethoxyphenylpropane-8,9-dio(l 10),3,4,5-trimethoxyphenyl 1-α-glucofuranoside(11),and benzyl-β-D-glucoside(12).Among these compounds,compound 1 exhibited some cytotoxicity in HepG2 cells.Conclusion The identified compounds mainly contained 2 tritepenoids,2 alkaloids,3 lignins,one unsaturated fatty acid,and 4 aromatic compounds.Compounds 3,8,10,11 were isolated from Lonicera genus for the first time,and 1,2,4,6,7 and 12 were isolated from the species for the first time.