日本落叶松胚状体干化处理对萌发的影响

Effect of Desiccation on Germination of Larix kaempferi Embryoids

[目的]提高日本落叶松胚状体的萌发能力,优化日本落叶松良种繁育技术。[方法]本研究根据子叶数量和胚轴形态,对胚状体进行分类,并分别统计其萌发率;利用“滤纸容器法”对类型Ⅰ胚状体进行干化处理,统计其萌发情况。[结果]胚状体分为10种类型。类型Ⅰ胚状体萌发率为4.48%,其余类型胚状体难以萌发。干化处理14 d后,类型Ⅰ胚状体萌发率提高到69.43%,且具有干化响应的胚状体更易萌发。[结论]本研究表明日本落叶松胚状体干化处理后萌发率明显提高,为该物种良种繁育提供依据。

[Objective]To improve the germination ability of Larix kaempferi embryoids and optimize the breeding technology of Larix kaempferi.[Method]In this study,we conducted classification on embryoids based on the number of cotyledon and the hypocotyl shape.Subsequently,their germination rates were recorded respectively.The germination rate of typeⅠembryoids was evaluated after desiccation using“paper vessel method”.[Result]Type I embryoids exhibited an initial germination rate of 5%,while the remaining types did not show any germination.However,after drying for two weeks,the germination rate of type I embryoids significantly increased to 69.43%,and embryoids with desiccation response were more likely to germinate.[Conclusion]These results show that the germination rate of Larix kaempferi embryoids is significantly improved after desiccation,which provides insights into new strategies for improving seedling production for this species.