胰岛素增敏剂罗格列酮升高血液中镍纹样蛋白水平

The increasement of blood METRNL protein by insulin sensitizer rosiglitazone

目的 探讨胰岛素增敏剂罗格列酮对血液中镍纹样蛋白(METRNL)水平的影响。方法 高脂饮食3个月诱导胰岛素抵抗的肥胖小鼠,给予罗格列酮治疗1个月,葡萄糖耐量实验检测罗格列酮对小鼠糖耐量的作用,酶联免疫吸附实验检测血清中METRNL水平,实时定量PCR检测肌肉、肝脏、白色脂肪、棕色脂肪、脑、脾脏、肾脏等组织中METRNL的表达,以及棕色脂肪中线粒体蛋白的表达。结果 罗格列酮治疗改善了高脂饮食动物的糖耐量,同时血液中METRNL浓度也显著增高;罗格列酮治疗增加了棕色脂肪和肾脏组织中METRNL的表达,对肌肉、肝脏、白色脂肪、脑、脾脏的METRNL表达没有影响;罗格列酮治疗增加了棕色脂肪线粒体相关蛋白的表达。结论 胰岛素增敏剂罗格利酮可能通过提高棕色脂肪和肾组织的METRNL表达升高血清METRNL水平,提示METRNL可能参与了罗格列酮对糖尿病的治疗作用。

Objective To investigate the effect of insulin sensitizer rosiglitazone on blood METRNL levels.Methods After fed with high fat diet(HFD)for 3 months,obese mice were treated with rosiglitazone for 1 month.Glucose tolerance was tested with glucose tolerance test(GTT),and METRNL levels in blood were measured by ELISA.Real time fluorescence quantitative PCR was used to detect the expression of METRNL in various tissues such as muscle,liver,white fat,brown fat,brain,spleen and kidney,as well as the expression of mitochondrial proteins in brown adipose tissue.Results Glucose tolerance of animals fed a high-fat diet was improved in rosiglitazone group,and blood METRNL levels were also increased significantly in this group.Rosiglitazone treatment increased the expression of METRNL in brown fat and kidney tissue.There was no effect on METRNL expression in muscle,liver,white fat,brain and spleen.Rosiglitazone increased the expression of mitochondrial-associated proteins in brown adipose tissue.Conclusion The insulin sensitizer rosiglitazone might increase the serum METRNL level by increasing the METRNL expression in brown fat and kidney tissue,suggesting that METRNL may be involved in the therapeutic effect of rosiglitazone on diabetes.