期刊文献+

MiR-29a/b/c对葡萄膜黑色素瘤细胞功能的影响及机制

The role of miR-29a/b/c in uveal melanoma cells and its underlying mechanism
下载PDF
导出
摘要 目的:探索miR-29a/b/c对葡萄膜黑色素瘤(UM)细胞功能的影响及其作用机制。方法:使用脂质体转染技术将hsa-miR-29a/b/c或基质金属蛋白酶2(MMP2)特异性siRNA转染进UM细胞。使用MTS实验和Matrigel Transwell实验分别检测UM细胞增殖和侵袭能力的变化。使用半定量PCR实验检测UM细胞中MMP2m RNA水平表达量的变化。使用ELISA实验检测UM细胞上清中MMP2蛋白分泌量的变化。结果:转染miR-29a/b/c抑制了UM细胞的增殖(P<0.05)和侵袭能力(P<0.05)。MiR-29a/b/c不影响MMP2 mRNA转录,但均能抑制UM细胞分泌MMP2(P<0.05)。在UM细胞中干扰MMP2抑制UM细胞的侵袭能力(P<0.05)。结论:Mi R-29a/b/c抑制UM细胞的增殖和侵袭。MiR-29a/b/c减少其下游靶蛋白MMP2的分泌,在UM细胞中干扰MMP2抑制细胞侵袭。 Objective:To investigate the role of miR-29a/b/c in uveal melanoma(UM)cells and the related mechanism.Methods:Hsa-miR-29a/b/c or matrix metalloproteinase 2(MMP2)-specific siRNA were transfected into UM cells by lipofectamine reagent.The proliferative and invasive potential of UM cells were evaluated by MTS and Matrigel Transwell assay respectively.MMP2 mRNA level was detected by Semi-quantitative PCR assay,and ELISA assay was used to measure the MMP2 secretion in the cell culture of supernatant of UM cells.Results:Transfection of miR-29a/b/c inhibited the proliferation(P<0.05)and invasive ability(P<0.05)of UM cells.MiR-29a/b/c could inhibit the expression of MMP2 at the protein level(P<0.05)instead of the mRNA level.Interfering MMP2 in UM cells inhibited the invasive ability(P<0.05)of UM cells.Conclusion:MiR-29a/b/c inhibits the proliferative and invasive ability of UM cells.MiR-29a/b/c reduces the secretion of its downstream target protein MMP2,and the knockdown MMP2 in UM cells inhibits cell invasion.
作者 潘虹婷 王超 朱鲜 侯强 PAN Hongting;WANG Chao;ZHU Xian;HOU Qiang(School of Laboratory Medicine and Life Sciences,Wenzhou Medical University,Wenzhou 325035,China;State Key Laboratory of Optometry and Visual Science,Eye Hospital,Wenzhou Medical University,Wenzhou 325027,China;Department of Pharmacy,Qionghai People’s Hospital,Qionghai 571400,China)
出处 《温州医科大学学报》 CAS 2024年第4期274-278,286,共6页 Journal of Wenzhou Medical University
基金 浙江省自然科学基金探索项目(LY21H120006)。
关键词 miR-29a/b/c 葡萄膜黑色素瘤 细胞增殖 侵袭 基质金属蛋白酶2 miR-29a/b/c uveal melanoma proliferation invasion matrix metalloproteinase 2
  • 引文网络
  • 相关文献

参考文献2

二级参考文献5

共引文献8

;
使用帮助 返回顶部