基于“脑肠轴理论”探讨龟羚帕安丸对PD大鼠纹状体DA及血清CCK、VIP水平影响的研究

Based on the Theory of Brain-gut Axis,to Explore the Effects of Guiling Pa'an Pill on Striatal DA and Serum CCK and VIP Levels in PD Rats

目的:基于“脑肠轴理论”探讨龟羚帕安丸对PD大鼠纹状体裂解液多巴胺(Dopamine,DA)、血清胆囊收缩素(cholecystokinin,CCK)及血管活性肠肽(vasoactive intestinal peptide,VIP)水平的影响。方法:采用6-羟基多巴胺注射法制作PD大鼠模型,随机分为模型组、西药组、中药高、中、低剂量组,每组15只,另设15只假手术组。模型组及假手术组给予等容积生理盐水灌胃,其余组给予相应药物灌胃,连续给药4周。酶联免疫吸附测定法(ELISA)检测纹状体裂解液DA及血清CCK、VIP水平。结果:模型组大鼠纹状体DA、血清CCK及VIP水平显著降低;中药各剂量组、西药组大鼠纹状体DA、血清CCK及VIP水平明显均增加(P<0.01)。结论:龟羚帕安丸具有明显神经保护作用,作用机制与激活脑肠轴、增加血清CCK及VIP水平、增加中脑黑质纹状体DA的水平、发挥改善肠道功能作用有关。

Objective Based on the brain-gut axis theory,the effects of Guilingpa'an Pill on the levels of dopamine(Dopamine,DA)in striatum lysate and serum cholecystokinin(cholecystokinin,CCK)and vasoactive intestinal peptide(vasoactive intestinal peptide,VIP)in PD rats were studied.Methods The PD rat model was made by injection of 6-hydroxydopamine(6-OHDA).The successful PD rats were randomly divided into model group(n=15),western medicine group(n=15),high dose group(n=15),middle dose group(n=15)and low dose group(n=15).The model group and sham operation group were given the same volume of normal saline,and the other groups were given corresponding drugs for 4weeks.Blood samples were taken from abdominal aorta of rats,and the levels of DA in striatum lysate and serum CCK and VIP were detected by enzyme-linked immunosorbent assay(ELISA).Results Compared with sham operation group,the levels of striatum DA,serum CCK and VIP in model group,western medicine group and each dose group were significantly lower than those in sham operation group(P<0.01);Compared with the model group,the levels of striatum DA,serum CCK and VIP in the western medicine group and each dose group were significantly higher than those in the model group(P<0.01).Conclusion Guiling Pa'an Pill has obvious neuroprotective effect,and its mechanism is related to activating brain-gut axis,increasing the level of CCK and VIP,increasing the level of DA in substantia nigra and striatum of midbrain,and improving intestinal function.