茶树春梢萌发早晚关联基因CsAL1的CAPS分子标记开发

The Development of CAPS Molecular Markers for CsAL1,A Gene Associated with Early and Late Spring Tip Emergence in Tea Plants

茶树(Camelliasinensis)作为一种叶用型经济植物,春梢萌发时间是关系茶叶经济价值的重要生物学性状。因此,选育茶树早生品种,对提升茶叶品质和经济效益具有重要的现实意义。以铁观音为参考基因组,通过全基因组关联分析,筛选到一个与茶树春梢萌发早晚高度相关的基因CsAL1(Auxilin-like 1,TGY040711)。运用SNP calling获得CsAL1各样本的SNPs,将SNPs与其春梢萌发表型进行关联分析,获得与早生性状显著相关的SNP位点。分析各SNPs的酶切位点,选择合适位点开发茶树早生性状相关CAPS分子标记。利用标记对12份茶树材料基因组DNA进行PCR扩增和酶切检测,随后在72份茶树材料中进一步验证,以期借助CAPS分子标记技术为探究CsAL1中单碱基位点突变与茶树早生性状的联系提供参考,为茶树早生品种的选育提供理论支撑。

Camellia sinensis is an economically important foliar plant.The time of spring sprouting is a crucial biological trait that affects the economic value of tea.Therefore,selecting and breeding early sprouting tea cultivars are of great practical significance for improving the quality and economic benefits of tea.The study used‘Tieguanyin’as the reference genome and screened a gene,CsAL1(Auxilin-like 1,TGY040711),which was highly significantly correlated with the time of spring sprouting based on genome-wide association analysis.SNP calling was used to obtain SNPs of CsAL1 in each sample.Correlation analysis of the SNPs and spring sprouting phenotypes was performed to obtain the key SNP loci that associating with spring sprouting traits.Suitable enzyme cleavage sites were analyzed for each SNP to develop CAPS molecular markers related to early-sprouting traits in tea plants.PCR was used to amplify the CAPS molecular markers,which were then digested in the genomic DNA of 12 tea materials.The markers were further verified in 72 tea materials to provide a reference for the association between single-base mutations in CsAL1 and early sprouting traits using CAPS molecular markers.This study also provided theoretical support for the breeding of early sprouting tea cultivars.