神丹6号绿壳蛋鸡和海兰褐蛋鸡产蛋期腹脂和血清脂代谢指标的比较研究

Abdominal fat and serum lipid metabolism of Shendan No6 green shell egg layer and Hy-line brown egg layer at laying stage

旨在比较神丹6号绿壳蛋鸡和海兰褐蛋鸡产蛋期腹脂和血脂代谢情况,为神丹6号绿壳蛋鸡的产蛋期饲养管理和营养调控提供理论依据。神丹6号绿壳蛋鸡和海兰褐蛋鸡19周龄商品代鸡,饲养至60周龄,19~28周龄每周采血一次,29~40周龄每2周采血一次,41~60周龄每4周采血一次,测量血清中脂质代谢相关生化指标,进行差异分析和相关性分析。结果显示:随着周龄增长,神丹6号绿壳蛋鸡腹脂率和肝脏指数变化不显著;血清甘油三酯(TG)、游离脂肪酸(FFA)和高密度脂蛋白(HDL)含量先升高后降低,之后变化不显著或波动变化;低密度脂蛋白(LDL)先降低后升高,之后变化不显著;总胆固醇(TC)含量整体降低;极低密度脂蛋白y(VLDLy)和卵黄蛋白原(VTG)含量前期变化不显著,之后波动变化。随着周龄增长,海兰褐蛋鸡血清HDL、LDL、VLDLy和VTG含量变化与神丹6号绿壳蛋鸡趋势略有差异,其他指标趋势基本一致。与海兰褐蛋鸡相比,神丹6号绿壳蛋鸡血清FFA和VTG含量前期较低,后期均与其含量接近,TG、TC、HDL、LDL和VLDLy含量则整体相近。产蛋前期(19周龄)、高峰期(28周龄)与后期(52周龄)比较分析结果显示,脂代谢指标中TG和FFA在神丹6号绿壳蛋鸡产蛋高峰期显著高于前期和后期(P<0.05),HDL和VTG均是后期显著低于前期(P<0.05)。综合分析表明,神丹6号绿壳蛋鸡产蛋期血脂代谢稳定,与海兰褐蛋鸡相比前期慢,后期相当。

In the current study,the abdominal fat and serum lipid metabolism of Shendan No 6 green shell egg layer and Hy-line brown egg layer at laying stage were compared in order to provide a theoretical basis for the feeding and management at laying stage and nutrient reg-ulation of Shendan No 6 green shell egg layer.The Shendan No 6 green shell egg layers and the Hy-line brown egg layers of 19 weeks old were raised to 60 weeks of age.During the treatment,blood samples were collected from the hens every week in their 19-28 weeks of age,every 2 weeks in their 29-40 weeks of age,and every 4 weeks in their 41-60 weeks of age.Then,serum lipid metabolic indices of the birds were measured,and the variance and correlation of the indices were analyzed.The results showed that,from 19 to 60 weeks of age,the per-centage of abdominal fat and liver index of the Shendan No 6 green shell egg layers generally remained unchanged;their serum triglyceride(TG),free fatty acids(FFA)and high-density lipoprotein(HDL)levels firstly increased and then decreased,and then remained un-changed or fluctuated;their low-density lipoprotein(LDL)level decreased first and then increased,and then remained unchanged;their to-tal cholesterol(TC)level decreased overall;and their yolk-targeted very-low density lipoprotein(VLDLy)and vitellogenin(VTG)levels remained unchanged first and then fluctuated.From 19 to 60 weeks of age,the trends of the serum HDL,LDL,VLDLy and VTG levels of the Hy-line brown egg layers were slightly different from those of the Shendan No 6 green shell egg layers,the trends of the other indices were generally the same.Compared with those of the Hy-line brown egg layers,the serum FFA and VTG levels of the Shendan No 6 green shell egg layers were lower early,and then they were almost the same.Their TG,TC,HDL,LDL and VLDLy levels were almost the same with those of the Hy-line brown egg layers.The comparative analysis of the early(19 weeks of age),peak(28 weeks of age)and late stages(52 weeks of age)showed that,in the lipid metabolic indices,TG and FFA at the peak stage were significantly higher than those at early and late stages in the Shendan No 6 green shell egg layers(P<0.05).HDL and VTG were significantly lower at the late stage than those at the early stage(P<0.05).The results indicated that the serum lipids of Shendan No 6 green shell egg layers metabolized well at the laying stage.And their metabolism was slower than that of the Hy-line brown egg layers early and then the same.