子痫前期患者血清腺苷活化蛋白激酶水平与可溶性血管内皮生长因子受体-1及氧化应激的相关性

Analysis of Correlation Between Serum Adenosine Monophosphate Activated Protein Kinase and Soluble Fms-1ike Tyrosine Kinase 1 Levels and Oxidative Stress in Patients with Preeclampsia

目的 分析子痫前期(PE)患者血清腺苷活化蛋白激酶(AMPK)水平与可溶性血管内皮生长因子受体-1(sFlt-1)及氧化应激的相关性。方法 选取2020年6月至2023年4月在成都医学院第一附属医院治疗的67例PE患者为试验组,选择本院同期住院的正常分娩孕妇50名为对照组。比较两组AMPK、sFlt-1和氧化应激反应水平[丙二醛(MDA)、超氧化物歧化物(SOD)];分析试验组不同严重程度的PE患者AMPK、sFlt-1、MDA、SOD水平差异;利用多变量逻辑回归方法对妊娠女性发病风险因子进行分析;分析试验组患者AMPK、sFlt-1与氧化应激的相关性。结果 试验组患者AMPK、sFlt-1、MDA水平均高于对照组,SOD水平低于对照组(P<0.05);轻度PE组的AMPK、sFlt-1、MDA水平均低于重度PE组,SOD高于重度PE组(P<0.05);两组妊娠期患糖尿病情况和AMPK、sFlt-1、MDA、SOD水平比较,差异有统计学意义(P<0.05);经多元Logistic回归分析显示,妊娠期合并糖尿病和AMPK、sFlt-1、MDA、SOD水平是影响孕妇发生PE的危险因素(P<0.05);Pearson相关性分析显示,PE患者AMPK、sFlt-1与MDA水平呈正相关,与SOD水平呈负相关(P<0.05)。结论 PE患者AMPK、sFlt-1水平出现异常升高,AMPK、sFlt-1水平与氧化应激指标存在一定相关性。

Objective To analyze the correlation between serum adenosine monophosphate activated protein kinase(AMPK)and soluble fms-1ike tyrosine kinase l(sFlt-1)levels and oxidative stress in patients with preeclampsia(PE).Methods Sixty-seven pregnant women with PE who were treated in the First Affiliated Hospital of Chengdu Medical College from June 2020 to April 2023 were included in the experimental group,and 50 healthy pregnant women who gave birth in the hospital during the same period were selected as the control group.Levels of AMPK,sFlt-1 and oxidative stress reaction indicators[malondialdehyde(MDA)and superoxide dismutase(SOD)]were compared between the two groups.The levels of AMPK,sFlt-1,MDA and SOD in PE patients with different severity were analyzed.Multiple Logistic regression analysis was used to analyze the risk factors of PE in pregnant women.The correlation between serum AMPK,sFlt-1 levels and oxidative stress in the experimental group was analyzed.Results The levels of AMPK,sFlt-1 and MDA in the experimental group were higher than those in the control group,while the level of SOD in the experimental group was lower than that in the control group(P<0.05).The levels of AMPK,sFlt-1 and MDA in the mild PE group were lower than those in the severe PE group,while the level of SOD in the mild PE group was higher than that in the severe PE group(P<0.05).There were significant differences in the presence of gestational diabetes mellitus,and levels of AMPK,sFlt-1,MDA and SOD between the experimental group and the control group(P<0.05).Multiple Logistic regression analysis showed that gestational diabetes mellitus,and levels of AMPK,sFlt-1,MDA and SOD were risk factors for PE in pregnant women(P<0.05).Pearson correlation analysis showed that AMPK and sFlt-1 levels were positively correlated with MDA level,and negatively correlated with SOD level in PE patients(P<0.05).Conclusion There is an abnormal increase in AMPK and sFlt-1 levels in PE patients.And there is a certain correlation between AMPK and sFlt-1 levels and oxidative stress indicators in PE patients.